摘要
[目的]以体外培养SD大鼠肺泡巨噬细胞 (AM)为靶细胞 ,研究PM10 与气传真菌单独和联合染毒对它的非特异性免疫功能的影响 ,并阐明剂量_反应关系。[方法]采集室内PM10、气传真菌 ,配制成染毒溶液。用不同浓度的PM10、气传真菌 ,以及PM10 和气传真菌联合染毒液染毒大鼠肺泡巨噬细胞 ,分别测定培养液中肺泡细胞存活率、吞噬率和吞噬指数以及细胞因子TNF_α ,观察PM10 与气传真菌单独和联合染毒对肺巨噬细胞存活率及其吞噬功能的影响。[结果]PM10 和真菌均能引起AM存活率降低 ,且有一定剂量_效应关系 ,剂量越高 ,存活率越低 (P<0.05)。联合染毒组存活率低于相应单独染毒组。2×2析因试验设计的方差分析结果显示 ,与对照组相比 ,联合染毒组存活率下降 ,且具有显著性意义 (P<0.01)。提示PM10 和真菌对巨噬细胞存活率的影响存在联合作用。单独和联合染毒组的吞噬率、吞噬指数也均呈剂量依赖性下降。高剂量组的吞噬率、吞噬指数远远低于阴性对照组 (P<0.05)。2×2析因试验设计方差分析结果表明 ,联合染毒组的吞噬率、吞噬指数与对照组相比 ,差异均有显著性意义 (P<0.01) ,提示PM10 和真菌对AM吞噬功能具有一定联合效应。结果还表明 ,阴性对照组培养上清液中未检测到TNF_α。染毒组TNF_α活性则随剂量上升而上升 (
To study the cytotoxic effects of indoor airborne PM10 and airborne fungi on alveolar macrophage (AM). Alveolar macrophages of Sprague_Dawley rat were served in vitro as target cells to observe the effects on the phagocytosis of alveolar macrophages and the levels of TNF_α after exposure to PM10 and airborne fungi either alone or both. The phagocytosis of alveolar macrophages was shown a significant dose_dependent reduction in the PM10 group,fungi group and both combined exposed groups,indicated by phagocytization rate and index. The activities of TNF_α in all exposed groups increased with the increase of exposed levels. There was a somewhat decline in the maximum_dose exposed group. The activity of TNF_α in combined exposed group was equivalent to the sum of PM10 and airborne fungi individually exposed group. The results indicated there was an additive effect of PM10 and airborne fungi. [Conclusion] The results suggested that indoor PM10 and airborne fungi have cytotoxicity on alveolar macrophages,injuring the phagocytosis and inducing alveolar macrophages to produce TNF_α.
出处
《环境与职业医学》
CAS
北大核心
2003年第4期257-260,共4页
Journal of Environmental and Occupational Medicine
基金
卫生部科技基金资助项目 (编号 :98-1-167)
