摘要
目的:加味升降散对糖尿病大鼠足细胞肾小球细胞黏附分子受体抗原(nephrin)、肾小球足细胞跨膜蛋白(podocin表达的影响,探讨其肾脏保护机制。方法:60只雄性成年SD大鼠随机分为空白组、模型组、培哚普利组、低剂量中药组和高剂量中药组。采用大鼠左侧切除左肾手术+高脂高糖饲料+链脲佐菌素(Streptozocin,STZ)法制备糖尿病大鼠模型。高剂量中药组大鼠给予27 g/(kg·d)加味升降散灌胃,低量中药组大鼠给予9 g/(kg·d)加味升降散灌胃,培哚普利组大鼠给予0.48 mg/(kg·d)(溶于2 mL的CMC-Na溶液)培哚普利灌胃。空白组和模型组给予等体积生理盐水灌胃。观察大鼠一般状况,应用考马斯亮蓝法监测24 h尿蛋白定量,应用全自动生化分析仪检测肾功能、血糖。光镜下观察大鼠肾脏病理学变化,免疫组织化法观察nephrin、podocin蛋白在肾脏组织的分布及表达情况。结果:与空白组比较,模型组血糖、血肌酐(Serum creatinine,SCr)、血尿素氮(Blood urea nitrogen,BUN)、24 h尿蛋白均升高(P <0.01),nephrin、podocin蛋白表达均下降(P <0.01);与模型组比较,培哚普利组及低、高剂量加味升降散血糖、SCr、BUN、24 h尿蛋白均下降(P <0.01);培哚普利组、低剂量中药组、高剂量中药组nephrin、podocin蛋白表达水平均有不同程度上调(P <0.05),其中高剂量中药组nephrin、podocin蛋白表达水平上调最为显著。结论:加味升降散能有效改善糖尿病大鼠肾功能,减少蛋白尿,改善肾小球硬化等,其途径可能与加味升降散上调大鼠足细胞nephrin、podocin蛋白表达水平,减轻大鼠足细胞损伤有关。
Objective:To discuss the effect of modified Shengjiang powder on the expression of glomerular cell adhesion molecule receptor antigen(nephrin)and glomerular podocyte transmembrane protein(podocin)in podocytes of diabetic rats,and to investigate its protection mechanism for kidney.Methods:Sixty male adult SD rats were randomly divided into the blank group,the model group,the perindopril group,the low-dose Chinese medicine group and the high-dose Chinese medicine group.Diabetic rat models were established through the nephrectomy on the left kidney,high-fat and high-sugar diets and Streptozotocin(STZ).The high-dose Chinese medicine group was treated with 27 g/(kg·d)modified Shengjiang powder;the low-dose Chinese medicine group was treated with 9 g/(kg·d)modified Shengjiang powder;the perindopril group was given 0.48 mg/(kg·d)(dissolved in 2 mL CMC-Na solution)perindopril by gavage;the blank group and the model group were administered with the same volume of saline by gavage.The general situation of rats were observed,the 24-hour urine protein was monitored by Coomassie Brilliant Blue Method,and the kidney function and blood glucose were detected by automatic biochemistry analyzer.The pathological changes in kidneys of rats under light microscope and the distribution and expression of nephrin and podocin in kidney tissues by immunohistochemistry were observed.Results:Compared with those in the blank group,the blood glucose,the serum creatinine(SCr),the blood urea nitrogen(BUN)and the 24-hour urinary protein in the model group were increased(P<0.01),the protein expression levels of nephrin and podocin were all decreased(P<0.01).Compared with those in the model group,the blood glucose,SCr,BUN and 24-hour urinary protein in the perindopril group and the low-dose and high-dose Chinese medicine groups were decreased(P<0.01).The protein expression levels of nephrin and podocin in the perindopril group and in the low-dose and high-dose Chinese medicine groups were all increased to some extent(P<0.05),and those in the hig
作者
张社峰
杨辰华
王沛人
ZHANG Shefeng;YANG Chenhua;WANG Peiren
出处
《新中医》
CAS
2019年第3期71-75,共5页
New Chinese Medicine
基金
河南省科技厅科技攻关项目(162102310374)
河南省省属科研机构预研专项(201704570)
