摘要
COP9信号小体(CSN)是进化上保守的蛋白复合体,在茉莉酸信号途径中起着重要的作用。橡胶树的乳管是一种特化的细胞器,是天然橡胶合成和储存的场所。现有的证据表明橡胶的生物合成可能受到茉莉酸信号途径的调控,但是对于茉莉酸信号途径调控天然橡胶的生物合成还了解的不够。本研究采用RACE技术结合RT-PCR从胶乳中克隆了8个CSN基因的全长cDNA序列,根据与拟南芥的相似性,分别命名为HbCSN1～HbCSN8。荧光定量PCR结果显示,8个CSN基因均能在树皮、不同发育时期的叶片、胶乳、雄花和雌花中表达,其中HbCSN5在胶乳中的表达量最高,其他成员在叶片中的表达量最高。而且,部分HbCSNs基因在胶乳中的表达受到割胶和茉莉酸甲酯处理的上调表达。因此,推测这些上调表达的成员可能参与胶乳的茉莉酸信号途径。
The COP9 signalosome(CSN)is an evolutionarily conservative protein complex and plays a crucial role in jasmonate(JA)signaling.The laticifer in rubber tree is specific for rubber biosynthesis.Although it is suggested that rubber biosynthesis in laticifer may be regulated by JA signaling,little is known about the regulatory mechanism of the JA signaling in rubber biosynthesis.In this study,the full-length cDNAs of eight CSN genes(designated as HbCSN1 to HbCSN8)which were respectively related to the eight CSN genes in Arabidopsis were cloned from the laticifer of rubber trees.The differentially expressed pattern of the eight CSN genes among bark tissues,leaves at the different development stages,latex,male and female flowers was revealed by real-time quantitative RT-PCR.The result showed that the eight HbCSNs were differentially expressed in all the tested tissues.Of which,HbCSN5 was the most abundance in latex,and the other HbCSNs were mainly expressed in the leaf.Additionally,most of the eight CSN genes in laticifer were up-regulated by tapping and methyl jasmonate(MeJA).The differentially responsive CSN members indicated that the COP9 signalosome may be involved in the JA signalings of latex in rubber trees.
作者
吴绍华
张世鑫
邓小敏
陈月异
田维敏
WU Shaohua;ZHANG Shixin;DENG Xiaomin;CHEN Yueyi;TIAN Weimin(Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Biology and Genetic Resources of Rubber Tree,Ministry of Agriculture and Rural Affairs/State Key Laboratory Breeding Base of Cultivation and Physiology for Tropical Crops,Haikou,Hainan 571101,China)
出处
《热带作物学报》
CSCD
北大核心
2019年第2期281-288,共8页
Chinese Journal of Tropical Crops
基金
国家天然橡胶产业技术体系育种技术与方法岗位科学家项目(No.CARS-34-YZ1)
中国热带农业科学院橡胶研究所基本科研业务费项目(No.1630022013004)
