摘要
目的探讨黄芪多糖(APS)对糖尿病模型大鼠的视网膜保护作用及其对血清胱抑素C的影响,为临床治疗提供依据。方法将60只SPF级Wistar大鼠随机分为对照组、模型组、APS组,每组20只,模型组与APS组采用腹腔注射链脲佐菌素(STZ)法建立糖尿病模型。造模结束后,APS组予以APS 400 mg/kg灌胃治疗,对照组和模型组予以等量生理盐水灌胃,连续10周后测量各组大鼠血糖、糖化血红蛋白、体质量,HE染色观察大鼠视网膜形态结构,Western blot法检测大鼠视网膜中肿瘤坏死因子-α(TNF-α)、血管内皮生长因子(VEGF)和p-Akt含量,ELISA法检测大鼠血清中血清胱抑素C水平。结果模型组及APS组大鼠血糖、糖化血红蛋白分别为(23.18±2.45)mmol/L、(20.19±3.76)%和(21.54±1.97)mmol/L、(18.36±3.01)%,高于对照组的(6.25±0.63)mmol/L、(4.63±1.19)%,体质量分别为(273.34±17.49)和(281.26±18.01)g,低于对照组的(343.57±21.46)g,差异均有统计学意义(P<0.05);APS组大鼠血糖、糖化血红蛋白低于模型组,体质量高于模型组,差异有统计学意义(P<0.05)。模型组大鼠视网膜细胞排列紊乱,内界膜肿胀,神经节细胞可见空泡、水肿样改变;APS组大鼠细胞排列较模型组规则,肿胀减轻,神经节内空泡较少。模型组和LPS组大鼠视网膜中血清胱抑素C表达量以及TNF-α、VEGF和p-Akt相对表达水平分别为(1.38±0.09)mg/L、(0.97±0.08)、(0.87±0.07)、(0.95±0.06)和(0.72±0.06)mg/L、(0.52±0.04)、(0.42±0.05)、(0.42±0.03),均高于对照组的(0.45±0.04)mg/L、(0.32±0.02)、(0.25±0.02)、(0.21±0.01),差异均有统计学意义(P<0.05);APS组血清胱抑素C表达量以及TNF-α、VEGF和p-Akt相对表达水平低于模型组,差异均有统计学意义(P<0.05)。结论黄芪多糖可以减轻糖尿病大鼠的视网膜病变,减少炎症因子及血清胱抑素C的表达,对糖尿病模型大鼠的视网膜有保护作用。
Objective To study and explore the protective effect of astragalus polysaccharides on diabetic rats and its effect on serum cystatin C,so as to provide the basis for clinical treatment.Methods 60 SPF Wistar rats were randomly divided into control group,model group,astragalus polysaccharides(APS)group,20 in each group.Streptozotocin(STZ)was injected intraperitoneally into model group and APS group.Diabetes model was established by injecting STZ intraperitoneally.The established diabetes rats were divided into APS group and model group.The APS group was treated with APS 400 mg/kg,and the model group and the control group were given the same amount of normal saline.After 10 weeks of continuous treatment,blood glucose,HbA1 c,and body weight were measured.Morphology of rat retina was observed by HE staining.Tumor necrosis factor-α(TNF-α)in rat retina was detected by western blot.Vascular endothelial growth factor(VEGF)and pAkt content,serum cystatin C levels were detected by ELISA method.Results The blood glucose and HbA1 c in the model group[(23.18±2.45)mmol/L,(20.19±3.76)%]and the APS group[(21.54±1.97)mmol/L,(18.36±3.01)%]were significantly higher than those in the control group[(6.25±0.63)mmol/L,(4.63±1.19)%],and the body weight was significantly lower than that in the control group[(273.34±17.49)g,(281.26±18.01)g vs.(343.57±21.46)g](P<0.05).The retinal cells in the model group were disordered and the inner boundary membrane was swollen.Vascular and edema-like changes were observed in the ganglion cells.The arrangement of cells in the APS group was more regular than that in the model group,swelling was reduced,and vacuoles in the ganglion were less.The expression level of serum cystatin C and the relative expression levels of TNF-α,VEGF and p-Akt in the retina of APS group were(1.38±0.09)mg/L,(0.97±0.08),(0.87±0.07)and(0.95±0.06),respectively,and those of the model group were(0.72±0.06)mg/L,(0.52±0.04),(0.42±0.05),and(0.42±0.03),respectively;the expression levels of serum cystatin C and the rel
作者
闫丰华
焦禄安
郑加军
许海
YAN Feng-hua;JIAO Lu-an;ZHENG Jia-jun;XU Hai(Ophthalmology Department of Rizhao Traditional Chinese Medicine Hospital,Rizhao,Shandong 276800;Huangjiahu Hospital of Hubei Traditional Chinese Medicine University,Wuhan,Hubei 430060,China)
出处
《热带医学杂志》
CAS
2019年第7期813-816,804,共5页
Journal of Tropical Medicine
基金
中国博士后科学基金(2013M542498)
