摘要
为获得端粒酶阳性肿瘤细胞特异表达载体用于癌症的基因治疗 ,克隆并构建了人端粒酶催化亚基 (hTERT)基因启动子调控的萤光素酶报告载体 .用脂质体转染法将其分别转染肿瘤细胞和正常细胞 ,检测其在肿瘤细胞和正常细胞中的转录活性 .hTERT启动子在所检测的 4种端粒酶阳性的肿瘤细胞中具有明显的转录活性 ,平均为阳性对照的 4 4 3% ;而在端粒酶阴性的正常人胚肺成纤维细胞中则无明显的转录活性 .提示hTRET启动子的转录活性在端粒酶阳性的肿瘤细胞中明显上调 。
In order to develop a telomerase specific expression system for the treatment of telomerase positive tumor cells,a luciferase expression vector (phTERT luc) drived by hTERT promoter was constructed. phTERT luc and its control vectors (pGL3 basic and pGL3 control) were transfected into tumor cell lines HepG2, Glc, A549, HeLa and normal fibroblast HEL by lipofectamine, respectively. The reporter gene luciferase activities were measured after transfection for 48 hours. hTERT promoter generated stronger activity in tumor cell lines with telomerase activity. There was no significant difference in transcriptional activity between the hTERT promoter and pGL3 basic plasmid in normal fibroblast HEL cell. The results indicated that the transcriptional activity was significantly up regulated in telomerase positive tumor cells, and suggested that telomerase specific expression vector afforded by hTERT promoter might be a novel and promising targeting approach for the treatment of tumors with telomerase activity.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
北大核心
2003年第4期533-536,共4页
Chinese Journal of Biochemistry and Molecular Biology
基金
国家自然科学基金资助项目 (No.3 0 0 70 85 9)
国家重大基础研究基金资助项目 (NO .G2 0 0 0 0 5 70 0 1)~~
