摘要
采用墨兰的茎尖和芽为外植体,研究墨兰的组织培养要点。通过对墨兰组织培养中外植体的选择、芽的诱导、继代增殖、壮苗培养、生根诱导和移植进行研究,运用正交试验筛选出芽快速生芽的最佳培养基为:MS+6 BA4.0mg·L-1+NAA1.0mg·L-1+琼脂8g·L-1+蔗糖30g·L-1,生根的最佳培养基为:1/2MS+NAA3.0mg·L-1。分析比较出芽长为1.0cm,叶长为1.5cm,叶数为2～3条的兰花苗有利于生根。
The explants of Cymbidium sinensis stems and spores were used to study Cymbidium sinensis tissue culture essentials. It dealed with the selection of explant, induction of proliferation, subculture, rooting and outplanting of Cymbidium sinensis.The results showed that the best medium to induce proliferation was MS+6BA 4.0 mg·L-1+NAA 1.0 mg·L-1+agar 8 g·L-1+sugar 30 g·L-1, by wielding orthogonal test. While rooting medium was half MS and 3.0 mg·L-1 of NAA. At the same time,we obtained by analyzing and contrasting the standard spore to bring about rooting, which offered basis of Cymbidium sinensis,such as enlarging produce, increasing living rate and improving economy benefits correspondingly.
出处
《林业科学研究》
CSCD
北大核心
2003年第4期434-438,共5页
Forest Research
基金
中国森林生态网络体系"线"的研究与示范(2002BA516A15)
关键词
墨兰
离体快繁
组织培养
外植体
培养基
试管苗
Cymbidium sinensis
spore induction
proliferation
rooting
outplanting
