摘要
目的 研究全反式维甲酸 (ATRA)对培养大鼠主动脉血管平滑肌细胞 (VSMC)增殖及细胞周期素E(Cy clinE)蛋白表达水平的影响。方法 血管平滑肌细胞采用组织贴块法培养。ATRA(2 .5× 10 - 6 mol·L- 1 )作用于经 2 0 %胎牛血清刺激后的VSMC ,2 4h后分别行3H TdR掺入实验测定和蛋白印迹检测CyclinE蛋白表达。 结果 ATRA明显抑制ATRA的DNA合成 (P <0 .0 1)和CyclinE蛋白的表达 (P <0 .0 5 )。结论 ATRA抑制CyclinE蛋白的表达是抑制VSMC增殖的原因之一。
Objective To investigate the effect of all trans retinoic acid(ATRA)on proliferation of cultural vascular smooth muscle cells (VSMC) and the expression of Cyclin E in rat.Methods The VSMC were cultured by explant method, VSMC stimulated by 20% fetal bovine serum were acted with ATRA(2.5×10 -6 mol·L -1 ).The expression of Cyclin E were determined by 3H TdR and Cyclin E Western Blotting after 24 h.Result ATRA obviously inhibits the synthesis of DNA ( P <0.01) and the expression of Cyclin E( P <0.05).Conclusion A cases of inhibition on ATRA proliferation of rat VSMC is inhibiting the expression of Cyclin E.
出处
《新乡医学院学报》
CAS
2003年第5期322-323,326,共3页
Journal of Xinxiang Medical University
关键词
全反式维甲酸
增殖
细胞
平滑肌
血管
细胞周期素
all trans retinoic acid
proliferation
cell
smooth muscle
vessel
Cyclin E
