摘要
目的 建立HPLC测定甲硝唑氯己定洗剂和复方氯己定含漱液中甲硝唑和葡萄糖酸氯己定含量的方法。方法 采用WatersC18色谱柱 ,以甲醇 水 三乙胺 (5 6∶4 4∶0 .1,用 5 0 %磷酸调pH至 3.0 )为流动相 ,检测波长 2 95nm。结果 两种成分能很好分离 ,甲硝唑和醋酸氯己定的线性范围分别为 10～ 2 9μg·mL-1(r =0 .9999)和 4 1～ 12 4 μg·mL-1(r =0 .9998) ;平均回收率分别为 99.6 %和 99.5 % ,RSD均小于 0 .9%。结论方法简便 ,结果准确可靠 ,适用于复方制剂中两组分的同时测定。
OBJECTIVE: To establish an external standard HPLC method for the determination of metronidazole and chlorhexidine in two compound preparations. METHODS: The Waters C18 column was used. The mobile phase was consisted of methanol-water-triethylamine(56:44: 0.1) (adjusted to pH of 3.0 by 50 % phosphoric acid). The UV detection wavelength was 295 nm. RESULTS: The two components had good resolution. The linear ranges were 10-29 μg · mL-1 for metronidazole and 41-124 μg · mL-1 for chlorhexidine acetate with the correlation coefficients 0.999 8 and 0. 999 9, respectively. The average recoveries were 99. 59 % and 99.5 % with RSD less than 0.9%. (n = 3). CONCLUSION: This method was simple and accurate, and suitable for the determination of two components in compound preparation.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2003年第7期532-534,共3页
Chinese Pharmaceutical Journal
