摘要
AIM: To investigate the putative role of human papillomavirus (HPV) infection in the carcinogenesis of esophageal squamous cell carcinoma in China.METHODS: Twenty-three esophageal squamous cell carcinoma samples and the distal normal epithelium from Shanxi Province, and 25 more esophageal squamous cell carcinoma samples from Anyang city, two areas with a high incidence of esophageal cancer in China, were detected for the existence of HPV-16 DNA by PCR, mRNA in situ hybridization (ISH) and immunohistochemistry (IHC) targeting HPV-16 E6 gene.
RESULTS: There were approximately 64 % (31/48) patients having HPV-16 DNA in tumor samples, among them nearly twothirds (19/31) samples were detected with mRNA expression of HPV-16 E6. However, in the normal esophageal epithelium from cancer patients, the DNA and mRNA of HPV-16 were found with much less rate: 34.7 % (8/23) and 26.1% (6/23) respectively.In addition, at protein level detected by IHC assay, 27.1% (13/48) tumor samples had virus oncoprotein E6 expression, while only one case of normal epithelium was found positive.CONCLUSION: HPV infection, especially type 16, should be considered as a risk factor for esophageal malignancies in China.
AIM:To investigate the putative role of human papillomavirus (HPV) infection in the carcinogenesis of esophageal squamous cell carcinoma in China. METHODS:Twenty-three esophageal squamous cell carcinoma samples and the distal normal epithelium from Shanxi Province,and 25 more esophageal squamous cell carcinoma samples from Anyang city,two areas with a high incidence of esophageal cancer in China,were detected for the existence of HPV-16 DNA by PCR,mRNA in situ hybridization (ISH) and immunohistochemistry (IHC) targeting HPV-16 E6 gene. RESULTS:There were approximately 64% (31/48) patients having HPV-16 DNA in tumor samples,among them nearly two- thirds (19/31) samples were detected with mRNA expression of HPV-16 E6.However,in the normal esophageal epithelium from cancer patients,the DNA and mRNA of HPV-16 were found with much less rate:34.7% (8/23) and 26.1% (6/23) respectively. In addition,at protein level detected by IHC assay,27.1% (13/ 48) tumor samples had virus oncoprotein E6 expression,while only one case of normal epithelium was found positive. CONCLUSION:HPV infection,especially type 16,should be considered as a risk factor for esophageal malignancies in China.
基金
grants from the National Natural Science Foundation of China,No.39925020
State Key Basic Research Program,No.G 1998051204
