摘要
从角蛋白酶产生菌株地衣芽孢杆菌L-25中提取基因组DNA,借助特定引物通过聚合酶链反应(PCR)扩增得到kerB基因片段.扩增后的kerB片段通过分子生物学方法克隆到产酶缺陷型枯草芽孢杆菌DB104菌株敏感细胞中进行表达.结果表明,表达成功的FD-8菌株(DB104/pLK18)可以在羽毛培养基上生长并完全水解羽毛.
A 1.4 kb keratinase gene( ker B) was isolated from Bacillus licheniformis L 25 strain by a polymerase chain reaction(PCR) with L 25 genomic DNA as the template. Amplified keratinase gene was directly cloned into a cloning vector PCR2.1.Then the keratinase gene within PCR2.1 was excised by restriction endonuclease XbaI SpeI , and inserted into the plasmid of PUB18 P43 for expressing. This new plasmid(PLK18) was transformed into protease deficient strain Bacillus subtilis DB104 competent cells. The FD 8(DB104/PLK18) strain could grow rapidly on feather medium and completely hydrolyze all feathers in the medium.
出处
《吉林大学学报（理学版）》
CAS
CSCD
北大核心
2003年第3期365-368,共4页
Journal of Jilin University:Science Edition
