摘要
分别用 2个不同限制性内切酶对猕猴桃属 2 7个种和 1 5个栽培品种的叶绿体基因 (rbcL基因和 psbA基因 )的PCR扩增产物进行酶切分析 ,共得到 2 5个限制性位点 ,其中 2 4个具有多态性。确立了该属植物的单元型分布 ,对该属植物的系统发育方式和部分重要种类的亲缘关系进行了探讨 ,拓展了可用于该属植物分子系统学研究的遗传信息。
Twentyseven species and fifteen variations of Actinidia were investigated through PCR-RFLP analysis of two chloroplast DNA fragments, including rbcL gene and psbA gene. The two chloroplast gene fragments were digested by two restriction endonucleasea respectively, which yielded twentyfive restriction sites and twentyfour of them were polymorphic. Haplotype of Actinidia was geographically established. Pattern of phylogene in Actinidia and relations between some important species were explored. Genetic information of molecular systematics in Actinidia was expanded by above all.
出处
《植物研究》
CAS
CSCD
北大核心
2003年第3期328-333,共6页
Bulletin of Botanical Research
基金
国家自然科学基金资助项目 ( 3 9960 0 0 7)
江西省自然科学基金资助项目 ( 0 2 3 0 0 0 1)
