摘要
番茄灰霉病菌在致病过程中能够产生 4种细胞壁降解酶 ,以PMG酶活性最高 ,其次是β 葡萄糖苷酶和PG酶 ,Cx最少。灰霉病菌在不同温度下侵染番茄叶片时产生的致病酶活性不同 ,4种酶在 2 0℃时表现了最高的活性 ,15℃次之 ,当温度达到 2 5℃时 ,各种酶的活性都急剧下降 ,随着温度的再升高 ,酶活更低。随着湿度的增高 ,病菌产生的细胞壁降解酶的活性也增加 ,当相对湿度达到 90 %以上时 ,4种酶的活性也达到最高。温湿度对番茄灰霉病菌产生细胞壁降解酶的影响趋势 。
Four kinds of cell wall degrading enzymes including carboxymethyl cellulose(Cx), polygalacturonase(PG),polymethyl galacturonate(PMG) and β glucosidase, which produced by Botrytis cinerea during the infection of tomato leaves,were investigated.PMG showed the highest activity during the pathogen infection, and the β glucosidase and PG were the next. The activities of these enzymes varied with the temperature during the infection, with the optimum for the highest enzyme activities is 25℃. Above 25℃,the enzyme activities decreased dramatically. Humidity also effected the production of these cell wall degrading enzymes.The enzyme activities increased with the increasing of relative humidity, and the highest activities appeared when the relative humidity was up to 90%. Our results showed that the effect of temperature and humidity on producing of cell wall degrading enzymes was consistent with that on disease development on tomato plants.
出处
《植物病理学报》
CAS
CSCD
北大核心
2003年第3期209-212,共4页
Acta Phytopathologica Sinica
基金
北京市自然科学基金重点项目 (60 0 1 0 0 2 )
国家"九五"攻关项目 (96 0 0 5 0 1 1 2 )
