摘要
目的 研究二巯基敌枯双对甲状腺的生物作用 ,探讨二巯基敌枯双是否属甲状腺激素干扰物。方法Wistar大鼠 60只 ,随机分为正常对照组 ( n=3 0 )和实验组 ( n=3 0 )。正常对照组给予溶剂二甲基亚砜 ,实验组给予二巯基敌枯双二甲基亚砜溶液 ,剂量为 5 0 m g/kg。于给药第 5 d、第 10 d和第 2 0 d,动物称重后分别放血处死两组动物。测定血清中 T4和 TSH的浓度 ;取动物甲状腺称重测定甲状腺脏器系数 ;常规 HE染色 ,光镜下观察组织学变化 ;用免疫酶法测定核增殖抗原 ( PCNA)在甲状腺中的阳性表达 ;用酶组织化学方法观察甲状腺琥珀酸脱氢酶( SDH)、甲状腺过氧化物酶 ( TPO)活性的变化。结果 实验组各期甲状腺脏器系数显著高于同期对照组 ( P<0 .0 5 ) ;实验组各期甲状腺滤泡上皮增生 ;实验组各期 PCNA表达阳性细胞数明显高于同期对照组 ( P<0 .0 5 ) ;实验组各期甲状腺滤泡上皮内 SDH和 TPO酶活性也明显增高 ( P<0 .0 5 )。
Objective To study the biological effects of thyroid induced by N′,N-methylene-bis (2-amino-5-sulfhydryl-1,3,4-thiadianole),discussing whether this agent is a thyroid hormone disruptor. Methods Sixty Wistar rats were divided into 2 groups, including one experiment group and one control. The rats of experiment group were fed with this agent at 50 mg/kg. Whole animals were weighed, then they were sacrificed by bleeding from femoral artery on the fifth,10th,20th day, respectively; at the same time, serum T 4 and TSH concentrations were examined and the thyroids were taken out and weighed for coefficient of thyroid gland weight; the pathological changes of thyroids were observed under light microscope; the enzyme activity of succinate dehydrogenase and thyroidperoxidase, and the positive expression of PCNA in thyroid gland were examined by histochemistry. Results Increased coefficient of thyroid gland weight,proliferation of thyroid epithelium,increased enzyme activity of SDH and TPO, and increased positive cells of PCNA were observed in the experiment group. Conclusion N′,N-methylene-bis (2-amino-5-sulfhydryl-1,3,4-thiadianole) is likely to be a thyroid hormone disruptor.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2003年第3期472-475,共4页
Journal of Sichuan University(Medical Sciences)
基金
国家自然科学基金重点课题 (批准号 L30 0 30 12 0 )
纽约中华医学基金资助
关键词
甲状腺激素干扰物
体内甄别实验
二巯基敌枯
组织化学
N′,N-methylene-bis (2-amino-5-sulfhydryl-1,3,4-thiadianole) Thyroid hormone disruptors Hyperplasia of thyroid gland Histochemistry
