摘要
大肠杆菌(E.coli)O157属于大肠杆菌的一个血清型,是引起出血性肠炎的主要病原体。山梨醇不发酵是目前进行E.coli O157筛选时的重要依据。本实验室用免疫法从冻鸡腿中分离出三株山梨醇发酵阳性的 E.coli O157,提示选用免疫法代替山梨醇发酵特征来进行 E.coli O157检测的必要性。
E. coli O157 do not ferment sorbitol whereas most other E. coli do and sorbitol Mac-conkey agar (SMAC) has been widely used for their isolation. However,SMAC medium relies entirely on differential sugar fermentation and therefore lacks sensitivity. Vidas (Vitek Immunodiaghostic Assay System) is an automated enzyme-linked fluoresent immunoassay (ELFA) for the detection of E. coli O157. We isolated 3 strains of E. coli O157 which ferment sorbitol from chicken legs imported from USA using Vidas. This result demonstrates the usefulness of Vidas in the rapid detection of E. coli O157 in food.
出处
《中国国境卫生检疫杂志》
CAS
1998年第2期85-87,127,共3页
Chinese Journal of Frontier Health and Quarantine
