摘要
目的 探讨中药复方“脊复康”对急性脊髓损伤大鼠运动功能恢复的影响。方法 90只雄性SD成年大鼠在无菌手术下,造成第12胸髓右侧半横断损伤模型。术后随机分成4组,即脊复康(J)组、激素(M)组、补阳还五汤(Y)组、空白对照(B)组。J组术后经食管插管逐日灌喂脊复康颗粒剂溶液。M组术后腹腔注射甲基强的松龙,隔日1次,共5次;Y组灌喂补阳还五汤煎剂;B组灌喂等量生理盐水。在术后1d、3d、7d、14d、28d,采用BBB法对各组大鼠的运动功能进行评分。在3d、7d、15d,从每组随机抽取5只,做病理学检查。另取6只为正常对照(N)组。结果 在脊髓半横断损伤的实验中,伤后3周,损伤诱发的运动功能障碍,可以在相当的程度上恢复达到正常水平。伤后3dJ组的BBB分值较M和B组的高(P>0.05);伤后7d,J组的BBB分值较B组高(P<0.05);伤后2周时,J组的BBB分值与N组没有差异,而M和B组的与N组的有统计学差异。结论 在大鼠脊髓半横断损伤的实验中,投给脊复康可以加速其运动功能的恢复,其机制有待于进一步的探讨。
Objective To investigate the effect of Chinese medicine formula,Jifukang,on motion function after acute spinal cord injury (ASCI) in rats Methods 90 SD rats with spinal cords injury at Ti2 were randomly divided into four groups, Jifukang treating group (J), Methylprednisoline treating group (M), Buyanghuanwutang treating group (Y), and control group with no treatment (B). The rats in J, Y, and B groups were feed with Jifukang,buyanghwutand and saline respectively, were the rats in M group were injected Methylprednisoline in peritoneal cavity. The BBB method is used to evaluate motion function after the injury for all groups at 1,3,7,14 and 28 days. At 3rd,7th and 15th day after the injury,5 rats were randomly killed for pathological study from each group,and 6 rats were selected as normal control group (N). Results At 3rd week after spinal injury in rat, it is possible that movement defect induced by ASCI was distinctly recovered and its power was nearly achieved normal level. Although the P > 0.05, BBB score in J group was clearly higher than that in M, Y and B groups respectively. The score in J group was significantly higher than other groups (P<0.05) at 7th day after the injury. At 14 day after the injury, the scores between J group and N group were not different (P > 0.05), but the score in M and B group is significantly low from N group( P < 0.05) . Conclusion The results show that 'Jifukang can advance functional recovery in movement defect cause by spinal cord injury. However, its mechanism needs to be further studied.
出处
《中国骨伤》
CAS
2003年第4期213-216,共4页
China Journal of Orthopaedics and Traumatology
基金
国家自然基金(编号:30171189)
