摘要
[目的]探讨检测血清IAP、sIL鄄2R和IL鄄12含量在大肠癌的辅助诊断、预后判断中的临床意义。[方法]采用单向免疫扩散法和ELISA法分别检测30例健康正常人及32例大肠癌患者手术前、后的血清IAP、sIL鄄2R和IL鄄12水平。[结果](1)大肠癌患者手术前血清IAP、sIL鄄2R水平均明显高于对照组(P<0.001和P<0.01),晚期组血清IAP、sIL鄄2R水平明显高于早期组(P<0.02和P<0.05),IAP与sIL鄄2R水平呈显著正相关(r=0.987,P<0.001);大肠癌患者血清IL鄄12水平明显低于对照组(P<0.05)。(2)串联IAP、sIL鄄2R两指标筛检大肠癌的敏感性为78.1%,特异性为93.33%,并联两指标筛检大肠癌,其敏感性为93.75%,特异性为73.3%。(3)手术后患者血清IAP、sIL鄄2R水平均明显低于手术前(P<0.001),早期组下降有明显差异性(P<0.001),而晚期组下降不明显(P>0.1);患者术后血清IL鄄12水平明显比术前升高(P<0.02),早期组上升显著(P<0.001),晚期组上升不明显(P>0.05)。[结论](1)检测患者血清IAP、sIL鄄2R水平对大肠癌的辅助诊断、病情判断、手术治疗和预后观察均有较高的临床价值。(2)联合检测血清IAP、sIL鄄2R对筛检大肠癌患者具有较高的敏感性。(3)血清IAP、sIL鄄2R和IL鄄12可作为指导大肠癌患者免疫学治疗较客观的指标。
To investigate serum levels of immunosupprcssive acidic protein(IAP),soluble interleukin-2receptor(sIL-2R)and interleukin-12(IL-12)in patients with colorectal cancer,and to explore their clinical signifi-cance for diagnosis and predicting the prognosis.Serum levels of IAP,sIL-2R and IL-12were measured in30normal controls and32colorectal cancer patients preoperatively and postoperatively with single immunodif-fusion and enzyme linked immunosorbent assay.(1)The preoperative serum levels of IAP and sIL-2R were significantly higher in32cases with colorectal cancer than those in normal controls(P<0.001,P<0.01).The levels of IAP and sIL-2R in advanced stage group were significantly higher than those of early stage group(P<0.02,P<0.05).There was an obvious correlation(r=0.98,P<0.001)between the levels of IAP and sIL-2R in preoperative pa-tients;The serum level of IL-12was significantly lower in32preoperative patients with colorectal cancer than that in normal controls(P<0.05).(2)When using the series target of IAP and sIL-2R to screen patients with colorectal cancer,the sensitivity was78.1%and specificity was93.33%,while only one of these two markers was considered,the sensitivity was93.75%and the specificity was73.3%.(3)The serum levels of IAP and sIL-2R decreased sig-nificantly after operation(P<0.001),but these changes were significant in early stage group(P<0.001),and were not in advanced stage group(P>0.1);The postoperative serum levels of IL-12increased significantly(P<0.02),but the changes were significant in early stage group(P<0.001),and were not in advanced stage group(P>0.05).[Conclu-sion]The serum levels of IAP and sIL-2R can be used for diagnosis,judging the malignant degree and the result of treatment,and predicting the prognosis in patients with colorectal cancer.Combinated deterimination of serum IAP and sIL-2R to screen patients with colorectal cancer had higher sensitivity.Serum IAP,sIL-2R and IL-12may be useful indicators in conducting immunotherapy in patients with colorectal cancer.
出处
《中国肿瘤》
CAS
2003年第7期412-414,共3页
China Cancer
基金
贵州省科技厅攻关项目(20011210)
关键词
大肠肿瘤
免疫抑制酸性蛋白
可溶性白细胞介素2受体
白细胞介素12
colorectal neoplasms
immunosuppressive acidic protein(IAP)
soluble interleukin-2receptor(sIL-2R)
interleukin-12(IL-12)
