摘要
通过对氮离子注入大豆产生的两个突变体及亲本过氧化物同工酶酶谱的比较研究,旨在明确其谱型特征及其分布,并通过酶谱差异探讨其变异程度和主要变异部位。电泳结果表明,两个突变体与其亲本的叶、茎、根过氧化物同工酶电泳图谱在谱带数量、位置、宽度和颜色深浅方面都具有明显差别。每一个样本都有各自特征的图谱类型,其中尤以根的谱带变化最大。因此用过氧化物同工酶作为变异依据之一是有一定价值的。
Peroxidase isozyme isoeletric focusing electrophoresis was used to identify the differences between the mutants induced by N+ implantation into soybean and that of their parent seedling. A mutant named 97 N+-D11 was obtained by N+ implantation in Jindou 11. Nitrogen cation was implanted into the third generation of 97 N+-D11. The new mutant was named as Ru N+-D11. The mutagenic effects on soybean was obvious. The results showed that N+ implantation could induce variations of the morphology, which included plant height, weight of individual plant, harvest exponent, colour and thickness of seed coat, protein and oil contents. The peroxidase isozyme patterns of all the mutants by N+ implantation were different from that of their parent, especially, in roots and stem. These differences in peroxidase isozyme patterns could be used as a useful predicting parameter for screening genotypes.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2003年第2期21-24,共4页
Chinese Journal of Oil Crop Sciences
基金
山西省自然科学基金资助项目(20001100)
