摘要
从健康家蚕、感染微粒子病家蚕,以及感染细菌性肠道病家蚕的消化道分离了200株肠球菌,并进行了数值分类学鉴定,以探讨家蚕消化道中肠球菌的生态学分布和家蚕微孢子与肠球菌的相互关系.研究结果表明:与健康蚕相比,4龄或5龄起蚕添食微孢子的微粒子病家蚕消化道内,肠球菌的数量分别增加5.5×105倍和0.74×102倍;菌种数从6个分别下降为3个和4个;生化表型数从27个分别下降为13个和14个;在菌种分布上,Ent.avium的分布频率从健康蚕的56%分别下降至20%和38%,而Ent.faecium的分布频率从健康蚕的2%分别上升至40%和16%;分离菌株共有59个生化表型.
Two hundred of enterococci strains were isolated from the intestine of healthy, pebrine and bacterial flacherie diseased silkworms and classified by using API STREP(V.50) system based on numerical taxonomy so as to inquire into the ecological distribution of enterococci in the intestine of silkworm and relationship between Nosema bombycis and enterococci. The results showed that, in comparison with that of the healthy silkworms, the number of enterococci in silkworms infected with Nosema bombycis after third or forth moulting increased by 5.5 x 105 and 0.744 x 102 times respectively.The number of the species decreased from 6 to 3 and 4.The number of biochemical polymorphism types decreased from 27 to 13 and 14.The distribution frequency of Enterococcus avium decreased from 56% to 20% and 38% , the distribution frequency of Enterococcus faecium increased from 2% to 40% and 16% respectively. There are 59 biochemical polymorphism types within the 200 enterococci strains.
出处
《蚕业科学》
CAS
CSCD
2003年第2期151-156,共6页
ACTA SERICOLOGICA SINICA
基金
国家自然科学基金(30070578)
浙江省自然科学基金(300254)