摘要
目的 探讨大鼠全脑缺血再灌注后不同时间对额叶神经细胞凋亡及P^(53)蛋白表达的影响。方法采用改良的Pulsineli 4-血管阻断(4-VO)方法建立SD大鼠急性全脑缺血模型,随机分为3组:正常组(n=7);假手术组(n=49);手术组(n=49)。缺血15min,分别于再灌注1、6、12、24、48、72h和7d断头取脑,采用TUNEL方法检测神经细胞凋亡,SP免疫组化方法观察额叶P^(53)蛋白的表达。结果 全脑缺血再灌注24h,可见少量TUNEL阳性细胞,再灌注48h可见较多TUNEL阳性细胞,72h出现大量TUNEL阳性细胞,7d明显减少。免疫组化染色:缺血组于再灌注24h可见少量P^(53)蛋白表达,48h达高峰,72h有所下降,7d明显下降,这种表达主要在细胞核内。结论 急性全脑缺血再灌注后的迟发性神经元坏死是以凋亡的方式发生的,全脑缺血再灌注后,额叶P^(53)蛋白表达增加,神经细胞凋亡和P^(53)蛋白的表达在一定时间内呈正相关。
Objective To investigate the effects on neuronal cell apoptosis of frontal lobe and P53 protein expression after global ischemia and reperfusion.Methods The acute global ischemia(15 min) model was produced by four- vessel occlusion as described by Pulsinelli in Sprague -Daewley rats, after cerebral ischemia, the rats randomly divided into three groups, that is normal group( n = 7 ) , sham - operative group( n = 49) , operative group(n= 49),all animals were decapitated in 1 、6、12、24、48、72h and 7d,respectively,after reperfusion.The neuronal cell apoptosis were detected by TUNEL method,meanwhile we utilized immunohitochemistry to observe the expression of r protein in frontal lobe. Results There were a few TUNEL - positive cells in 24h after ischemia and reperfusion, we observed more TUNEL - positive cells in 48h, this expression was maximal in 72h,7d after ischemia TUNEL- positive cells declined obviously.Immunohitochemistry staining:there were a few P53 protein expression in 24h in ischemia group.This expression was maximal in 48h, subsequently began to decline,a few of expression were observed at 7d after ischemia,The staining mainly found in the nuclei.Conclusion The delayed neuronal death after global ischemia and reperfusion was apoptosis,P53 protein expression was increased following global ischemia and reperfusion, the relationship between the neuronal cell apoptosis and P53 protein expression was a positive correlation in a certain time following global ischemia and reperfusion.
出处
《河南实用神经疾病杂志》
2003年第3期1-3,共3页
Henan Journal of Practical Nervous Diseases
