摘要
根据猪传染性胃肠炎病毒和猪呼吸道冠状病毒的S基因核苷酸序列 ,设计合成了2对引物 ,以猪传染性胃肠炎病毒和猪呼吸道冠状病毒细胞培养物为模板 ,进行PCR特异性片段扩增 ,猪传染性胃肠炎病毒扩增片段大小为886bp,猪呼吸道冠状病毒扩增片段大小为214bp。建立的套式PCR经过特异性、敏感性试验及对临床送检样品检测 ,证明本法具有快速、特异和高度敏感的特点。
Two pairs of primers were synthesized based on Sgene nucleotide sequences of transmissible qastroenteritis virus and porcine respiratory coronavirus.PCR was developed to amplify specifi squences,using TGEV and PRCV cell cultures as template.The amplified segment of TGEV gene was886bp and that of PRCV gene was214bp.Results showed that the n=pcr was rapid,specific and sensitive in detecting the clinical samples.
出处
《中国动物检疫》
CAS
2003年第7期21-23,共3页
China Animal Health Inspection
关键词
应用
套式PCR检测
猪
传染性胃肠炎病毒
呼吸道冠状病毒
试验
Transmissible gastroenteristis virus,Porcime repiratory coronatirus,Polymerase chain reaction,Detection
