摘要
目的:研究胎盘表皮生长因子受体(EGFR)蛋白及基因的表达在重度妊娠高血压综合征(妊高征)发病中的作用以及对妊高征胎儿生长发育的影响。方法:采用免疫组织化学法和逆转录-聚合酶链反应(RT-PCR)技术测定21例重度妊高征患者[分2组,重度妊高征合并胎儿宫内发育迟缓(IUGR)组9例和重度妊高征未合并IUGR组12例]胎盘EGFR的表达,并与15例正常妊娠者(对照组)比较。结果:与对照组相比,重度妊高征患者胎盘EGFR蛋白及其mRNA的表达显著降低(P<0.01),重度妊高征合并IUGR组与重度妊高征未合并IUGR组之间胎盘EGFR的表达差异无显著性(P>0.05)。结论:胎盘滋养层细胞EGFR表达显著降低,可能是重度妊高征发病的重要因素;如果EGFR的低表达发生较早,容易发生IUGR。
Objective: To study the expression of placental epidermal growth factor receptor (EGFR) and EGFR mRNA in severe pregnancy induced hypertension (PIH), and to explore the effect of EGFR on the pathogenesis of severe PIH and its fetal development in PIH. Methods: Twenty-one severe PIH (9 with Intrauterine growth retardation(lUGR)and 12 without IUGR) and 15 normal pregnancies (controls) were selected. The expression of EGFR in placenta was determined by immunohistological method, and the related quantity of EGFR mRNA was analysed by the method of reverse transcription-polymerase chain rection (RT-PCR) method. Results: Compared with the normal pregnancies, the expression of EGFR in placenta in the cases with severe PIH decreased significantly, and there was no significant difference of EGFR expression between the cases of PIH with and without IUGR. Conclusion: Decreased expression of EGFR may play a role in the pathogenesis of PIH. If expression of EGFR reduced earlier during the pregnancy, it would give forth IUGR.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2003年第4期312-314,F002,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省科技厅应用基础基金资助项目(BJ2000021)
关键词
妊娠并发症
心血管
高血压
胎儿宫内发育迟缓
受体
表皮生长因子
pregnancy complication, cardiovascular
hypertension
intrauterine growth retardation
receptor, epidermal growth factor
