摘要
目的 了解广西肝癌高、低发地区乙型肝炎病毒 (HBV)核心基因启动子突变株的流行特征。方法 用套式PCR(nPCR)扩增 77例广西原发性肝癌高发地区隆安县和低发地区桂林市人群HBV无症状携带者血清HBVDNA核心基因启动子 ,阳性者用直接测序法测序。结果 发现 77例HBsAg无症状携带者中 39例HBVDNA阳性 ,阳性率为 5 0 6 % (39 77)。隆安县标本HBVDNA阳性率为 5 5 6 % (2 0 36 ) ,其中 35 %标本核心基因启动子发生突变 ,常见的突变类型为双突变 (nt176 2A→T、nt 176 4G→A) ,占 2 5 % (5 2 0 ) ;桂林市标本HBVDNA阳性率为 4 6 3% (19 4 1) ,其中 4 7 4 %标本有突变 ,常见的突变类型也是双突变 ,占 4 19,两地区T1 76 2 A1 76 4突变株流行率差异无显著性 (P >0 0 5 )。结论 广西T1 76 2 A1 76 4突变株流行分布与肝癌发病率分布不成正相关关系。
Objective To understand the prevalence of HBV core promoter mutant (T 1762 A 1764 mutant) isolated from asymptomatic carriers from areas with higher and lower incidence of hepatocellular carcinoma (HCC) in Guangxi Methods A nested polymerase chain reaction (nPCR) was used for amplification of HBV DNA core promoter in sera, and then HBV DNA nPCR products were sequenced by direct sequencing Results The results show that 50 6% (39/77) of all HBV asymptomatic carriers were positive for HBV DNA HBV DNA positive rates of the samples from HCC higher incidence area, Longan County, and from lower incidence area, Guilin city were 55 6% (20/36) and 46 3% (19/41), respectively HBV core promoter mutants could be seen in 35% in Longan positive samples and 47 4% in Guilin The common mutations in both regions were all double mutations (nt 1,762 A→T; nt 1,764 G→A), accounting for 25% and 21%, respectively The difference of the double mutant between Longan County and Guilin city was not significant ( P >0 05) Conclusion These data implicated that the prevalence of HBV core promoter mutant isolated from asymptomatic carriers may not be correlated with the incidence of HCC in Guangxi
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2003年第2期140-142,共3页
Chinese Journal of Experimental and Clinical Virology
基金
卫生部 ( 98 1 3 5 1)
广西科技厅资助项目 (桂科回字981713 6)
