摘要
目的 分析乳腺癌病人外周血 T细胞亚群的变化情况 ,探讨乳腺癌病人机体的免疫状况。 方法 应用流式细胞仪测定 33例乳腺癌病人与 2 0例健康女性外周血 CD3+、CD4 +、CD8+百分比 ,CD4 + / CD8+比率。 结果 (1)乳腺癌病人外周血 CD3+、CD4 +显著低于对照组 (P<0 .0 5 )。 (2 ) 、 期乳腺癌 T细胞亚群无明显改变 ,且与对照组无明显差异 ; 、 期乳腺癌 CD3+ 、CD4 + 、CD4 + / CD8+ 显著低于 、 期 (P<0 .0 5 ) ,CD8+ 百分率显著高于 、 期 (P<0 .0 5 )。 (3)乳腺癌腋淋巴结转移组 (L+) CD3+ 、CD4 + 、CD4 + / CD8+ 显著低于无转移组 (L- ) (P<0 .0 5 ) ,CD8+ L(+)组显著高于 L (- )组 (P<0 .0 5 )。 结论 外周血 T细胞亚群的检测 ,对判断乳腺癌患者的病情、临床分期、预后以及机体的免疫功能的观察有一定意义。
Objective To analysis the alteration of peripheral blood T cell subset for the evaluation of the immunologic situation of the patients with breast cancer. Methods The percentages of CD\-3\++, CD\-4\++, CD\-8\++T cells and the ratio of CD\-4\++/CD\-8\++ in peripheral blood of 33 patients with breast cancer and 20 blood donors were determined by flow cytometry(FCM). Results (1)The percentages of CD\-3\++, CD\-4\++ cells in peripheral blood in patients with breast cancer were significantly lower than those in normal controls groups(P<0 05). (2)There was no obvious difference of T cell subset among the Ⅰ, Ⅱ stage breast cancer and the normal control group, while CD\-3\++, CD\-4\++, CD\-4\++/CD\-8\++ in Ⅲ, Ⅳ stages breast cancer were remarkably lower than those in Ⅰ, Ⅱ stages(P<0 05), however the percentage of CD\-8\++ cells was significantly higher than that in Ⅰ, Ⅱ stage(P<0 05). (3)The percentage of CD\-3\++, CD\-4\++and the ratio of CD\-4\++/CD\-8\++ in patients with metastasis of axillary lymph node(L+) were remarkably lower than those without metastasis of axillary lymph node(L-) (P<0 05), while the percentages of CD\-8\++ was significantly higher than L(+) (P<0 05). Conclusion Determination of T cell subset in peripheral blood in patients with breast cancer might be of some significance in the diagnosis of the state of the disease, clinical staging, evaluation of the prognosis and the state of immunity.
出处
《福建医科大学学报》
2003年第2期210-211,共2页
Journal of Fujian Medical University
