摘要
对云南省西双版纳地区 1 7种龙脑香科树种根系丛枝菌根 (Arbuscularmycorrhiza,AM)真菌的定居情况进行了调查 ,并对根围土壤中AM真菌进行了分离和鉴定。结果表明 ,调查根样均有不同程度的菌根感染 ,感染率最高可达 40 % ,调查揭示了西双版纳地区龙脑香科植物在自然条件下可形成丛枝菌根。初步从龙脑香科植物根际土壤中分离、鉴定出 32种AM真菌 ,隶属于无梗囊霉属 (Acaulospora)、球囊霉属 (Glomus)、原囊霉属 (Achaeospora)、拟球囊霉属 (Paraglomus)和盾巨孢囊霉属 (Scutellospora) ,其中 。
In order to investigate the status of arbuscular mycorrhizae (AM) in tropical dipterocarpaceae forest, rhizospheric soil with roots of seventeen species of dipterocarpaceae plants grown in both native forests and plantations in tropical region Xishuangbanna, Yunnan Province, were sampled to determine their mycorrhizal colonization, spore density, isolation frequency, relative abundance and species richness of AM fungi. The mycorrhizal colonization rate of host plants were measured under a BX50 Olympus Microscope with Automatic Photo Micrographic System (320_640×) after staining with 0.5% acid fuchsin. Spores of AM fungi in aliquots (20 ml) of each soil were isolated by wet_sieving decanting and cane sugar centrifugal method, which were used to identify according to the newest taxonomic system described by Morton and Redecker (2001). Species richness was measured as the number of species compared with the number of individuals in the community. Results showed that most roots collected were colonized by AM fungi, typical arbuscules and vesicles were formed with various colonization rates (up to 40%, grade VIII), and 0_1.86 vesicles per mm root length. Species richness on Parashorea chinensis was the highest (4.31), while Shorea assamica showed the lowest (1.63). Hopea mollissima gave the maximum spore density (7.76), Vatica astrotricha the minimal (3.09). Thirty_two AM fungal species belonging to the genera Acaulospora, Glomus, Paraglomus, Achaeospora and Scutellospora were identified. The frequencies and relative abundances of AM fungi varied. Acaulospora and Glomus were likely the dominant proportion in the rhizospheric soil of dipterocarpus plants in the tropical region. It was suggested that most dipterocarpus plants can form typical AM and there was high species diversity of AM fungi in tropical dipterocarpaceae forest. The functional role of these AM fungi in the physiology of dipterocarpus plants needs further research.
出处
《植物生态学报》
CAS
CSCD
北大核心
2003年第3期360-365,共6页
Chinese Journal of Plant Ecology
基金
国际热带木材组织 (ITTO)PD38/98项目 ( 2 0 0 1_2 0 0 3)
中国林业科学研究院基金重点课题2 0 0 1_0 3( 2 0 0 2_2 0 0 4 )
关键词
西双版纳地区
龙脑香科
植物
AM真菌
袍子密度
Dipterocarpaceae, Arbuscular mycorrhizae, Colonization status, Spore density, Species richness
