期刊文献+

FMDV OH99株基因组全序列的测定及其基因特征研究 被引量:3

The Sequence Analyses of Full-Length Genome of FMDV OH99 Strain
下载PDF
导出
摘要 采用RT-PCR方法对FMDV OH99株基因组全序列进行了分子克隆与测序。结果表明OH99株基因组全基因组序列长8040nt,其中5’NCR长1026nt,前导蛋白(L)编码区长603nt。该毒株结构蛋白与非结构蛋白编码区的核苷酸序列为6318nt,3’NCR长93nt,其后是poly(A)尾巴,测序结果表明该结构至少含有56个A。应用分子生物学软件,将OH99株与其它参考毒株进行了序列比较,并对其基因特征、推导的氨基酸序列进行了研究分析。结果显示,在分类地位上OH99株归属于O型FMDV,与OTY TW/97具有较高的同源性,而与其他参考毒株的差异性比较大,而且在基因组功能未知区域和3A编码区域具有两处明显的基因片段缺失现象,其中3A编码区缺失30nt,与OTY TW/97株相同,但功能未知区域的缺失状况与OTY TW/97稍有差异。根据VP1基因序列,对OH99株与参考毒株进行了系统发生树分析,分析结果表明OH99株与OTY TW/97株在同一基因型内,其遗传关系最近,而与其毒株遗传关系较远。 The full-length genome of Foot-and-Mouth disease virus (FMDV) OH99strain was cloned by RT-PCR and 3'RACE, and sequenced. The result shows that the full-length genome of OH99 strain is 8040nt in length, including 5'NCR, Leader protein coding region, Polyprotein coding region, and 3'NCR Their nucleotide sequence is 1026nt, 603nt, 6318nt, and 93nt respectively. Besides, poly (A) tail was also coined by 3'RACE, it's 56nt in length. The full-length genome of OH99 strain was compared with other reference strains, and its genome characteristic is also analyzed. With the help of some biological ware, The Phylogenetic tree obtained from the nucleotide sequence of VPlgene from OH99 strain and ten reference strains shows that OH99 strain could be ranked FMDV O style. Furthermore, this strain and OTY TW/97 are in the same genotype, their nucleotide sequence share high homology, but it shows remarkable divergence with other reference strains, including china99 strain. For example, two short genome segment was deleted in the function-unknown region and 3A coding region of OTY TW/97 and OH99 strain. Which would affect the host tropism of virus. The results suggest OH99 and OTY TW/97 may come from the same ancestor, but the genetic distance between OH99 and other reference strains is far.
出处 《中国病毒学》 CSCD 2003年第3期259-264,共6页 Virologica Sinica
基金 国家"973"项目资助(G1999011901)
关键词 FMDV OH99株 基因组 序列测定 特征 分子克隆 非编码区 结构蛋白 口蹄疫病毒 Foot-and-Mouth disease virus(FMDV) OH99 strain Non-coding Region Structural protein
  • 引文网络
  • 相关文献

参考文献10

  • 1Beard C W, Mason P W. Genetic determinants of altered virulence of Taiwan Residents foot-and-mouth disease virus [J]. J Virol, 2000, 74 ,987-991. 被引量:1
  • 2Joachim S, Alba G, Ernst B, et al. The structures of picomaviral proteinases[J]. J Virus Res,1999, 62: 159-168. 被引量:1
  • 3Rico-Hess E R, Pallansch M A, Nottay B K, et al. Genophic distribution of wild polivirus type I genetypes[J]. Virol, 1987, 160:311-322. 被引量:1
  • 4Rieder E, Bunch T, Brown F, et al. Genetically engineered foot and mouth disease virus with poly (C) tracts of two nuclentide are virulent in mice [J]. J Virol, 1993, 67:5139-5145. 被引量:1
  • 5Zibert A, Maass G, Strebel K, et al. Infectious foot and mouth disease virus derived from a cloned full-length cDNA [J]. J Virol, 1990, 64:2467-2473. 被引量:1
  • 6Lope de, Quinto S, Martinez-Salas E. Conserved structural motif located in distal loops of apthovirus IRES domain 3 are required for internal initiation of translation [J]. J Virol, 1997, 71:4174-4175. 被引量:1
  • 7Beard C W, Mason P W. Genetic determiants of altered virulence of Taiwan FMDV [J]. J Virol, 2000, 74:987-991. 被引量:1
  • 8Xiang W A, Cuconati D, Hope k, et al. Complete protein linkage map of polivirus P3 protein: interaction of polymerase 3Dpol with genetic variants of 3AB [J]. Virol, 1998, 72:6732-6741. 被引量:1
  • 9Saiz M, Gomez S, Martine-salas E. Deletion or substitution of the poliovirus 3NCR abrogate infectivity and virus replication [J]. J Gen Virol, 2001, 82:93-101. 被引量:1
  • 10Barry B, Marvin J G, Howard L B. The relation of poly (A) length to specific of viral RNA: A comparison of different types of foot and mouth disease virus [J]. Virol, 1979, 98:480-483. 被引量:1

同被引文献38

  • 1马鸣潇,金宁一,李昌,刘慧娟,郑敏,金明兰,贾雷立,张林,鲁会军,沈国顺,王瑞林,金扩世.口蹄疫病毒O/LZ株的分子特性分析[J].畜牧兽医学报,2006,37(10):1027-1035. 被引量:2
  • 2Emini E A, Hughes J V, Perlow D S, et al. Induction of hepatitis A virus-neutralizing antibody by a virus-specfic synthetic peptide [J]. J Virol,1985,55:836-839. 被引量:1
  • 3Jameson B A, Wolf H. The antigenic index: A novel algorithm for prediction antigenic determinants [J]. Comput Appl Biosci,1988, (4):181-186. 被引量:1
  • 4Lewis S A, Morgan D O, Grubman M J. Expression processing and assemb1y of FMDV capsid structures in heterologous systems: induction of neutralising antibody in pigs [J]. J Virol, 1991, 65:6572-6580. 被引量:1
  • 5van Lierop M J, Wagenaar J P, van Noort J M,et al. Sequences derived from the highly antigenic VP1 region 140 to l60 of FMDV do not prime for a bovine T-cel1 response against intact virus [J]. J Virol,1995, 69: 4511-4514. 被引量:1
  • 6Abrams C C, King A M Q, Belsham G J, et al. Assembly of FMDV empty capsids synthesized by a vaccinia virus expression system [J]. J Gen Virol,1995, 76:3089-3098. 被引量:1
  • 7Garnier J, Osguthorpe D J, Robson B. Analysis of the accuracy and implications of simple method for predicting the secondary structure of globular proteins [J]. J Mol Biol,1978,12:97-102. 被引量:1
  • 8Chou P Y, Fasman G D. Prediction of the secondary structure of protein conformation [M]. New York: Plenum Press, 1990.549-586. 被引量:1
  • 9Karplus P A, Schulz G. Prediction of chain flexibility in proteins [J]. Naturwissenschaften, 1985, 72:212-213. 被引量:1
  • 10Kyte J, Doolittle R F. A simple method for displaying the hydropathic character of a protein [J]. J Mol Biol, 1982, 157:105-132. 被引量:1

引证文献3

二级引证文献11

;
使用帮助 返回顶部