摘要
目的 探讨EBV感染、TNF -α与非霍奇金淋巴瘤 (NHL )组织坏死的相关性。方法 将 12 4例NHL标本分为坏死组与无坏死组 ;分成NK/T、TCL、BCL 3种类型。采用原位杂交方法检测EBV编码的RNA (EBER 1/2 ) ,对EBER 1/2阳性标本采用免疫组织化学S -P法检测TNF -α水平。结果 坏死组 2 7例 ,无坏死组 97例 ;NK /T 2 3例、TCL 5 0例、BCL 5 1例。EBER1/2阳性率为 2 8.2 % ( 35 /12 4) ,其中坏死组阳性率为 85 .2 % ( 2 3/2 7) ,无坏死组为 12 .4% ( 12 /97) ,2组比较有非常显著性差异 ,P <0 .0 1。NK /T感染率为 5 2 .2 % ( 12 /2 3) ,TCL为 36.0 % ( 18/5 0 ) ,BCL为 9.8% ( 5 /5 1) ,NK/T、TCL与BCL比较有非常显著性差异 ,P <0 .0 1。TNF -α阳性率为 8.6% ( 3/35 ) ,且多呈弱阳性。结论 EBV感染 (EBER 1/2阳性 )主要见于NK /T和TCL ,与NHL组织坏死相关 ;TNF -α与NHL组织坏死无明显相关性 ;
Objective To explore the association of the expression of TNF-α and the infection of EBV with tissue necrosis in NHLs.Methods The 124 cases of NHLs were divided into two groups with or without necrosis observed by light microscope.They were also seperated into NK/T cell lymphoma(NK/T)、T-cell lymphoma(TCL) and B-cell lymphoma(BCL) by immunophenotype.In situ hybridization(ISH) were performed to detect EBV-encoded small nuclear RNAs(EBER1/2).And the expression of TNF-α was dectected in the EBER1/2 positive cases by immunohistochemistry(IHC).Results There were 27 cases with tumor necrosis and 97 cases without.23 cases were tested as NK/T,50 cases as TCL,and 51 cases as BCL.The overall positive rate of EBER1/2 was 28.2%(35/124),with 85.2%(23/27) in group with tumor necrosis and 12.4%(12/97) in group without.The difference between the two groups was significant(P<0.01).The positive rate of EBER1/2 in NK/T、TCL、BCL was 52.2%(12/23)、36.0%(18/50) and 9.8%(5/51) respectively.The necrotic rate in NK/T and TCL was higher than that in BCL(P<0.01).The positive rate of TNF-α was 8.6%(3/35)in EBER1/2 positive group and the staining was quite faint.Conclusion The infection of EBV is mainly found in NK/T and TCL and may be related to the tissue necrosis in NHLs.TNF-α don′t corelated to the tissu necrosis in NHLs.Several mechanisms may be involved in inducing necrosis of tumor tissue by infection of EBV.
出处
《实用癌症杂志》
2003年第3期237-239,共3页
The Practical Journal of Cancer
基金
贵州省教育厅资助 (No :1 999- 4 77)
