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哮喘患儿T细胞亚群功能状态的研究

A STUDY OF T CELL PRODUCED CYTOKINE PANEL IN CHILDREN WITH ASTHMA
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摘要 为观察哮喘患儿T细胞亚群功能状态 ,以探讨其发病机制 ,分别采用酶联免疫吸附试验 (ELISA)、流式细胞术 (FCM)检测哮喘患儿外周血单个核细胞 (PBMC)培养上清液中IL 4、IFN γ及PBMC细胞膜上CD30的表达。结果显示 ,发作组PBMC培养上清液中IFN γ为 338 98±90 4 9ng/L ,IL 4为 37 75± 1 1 94ng/L ,IgE为 2 82 39± 83 33U/L ,与对照组比较差异有显著性意义 (P均 <0 0 1 ) ;经治疗后IFN γ为 4 0 8 6 9±93 5 8ng/L ,IL 4为 33 35±1 1 99ng/L ,IgE为 1 37 2 8± 89 0 9U/L ,与对照组比较差异有非常显著性意义 (P均 <0 0 1 ) ;IL 4与IgE呈正相关关系(r=0 5 6 1 ,P <0 0 5 ) ;哮喘患儿PBMCCD30表达增强。提示哮喘患儿存在T细胞亚群功能紊乱 ,Th1 To observe the state of balance between Th1/Th2 in children with asthma by assaying lymphocyte produced cytokines and the expression of CD30 membrane antigen on PBMC. The levels of IFN γ, IL 4 in PBMC and serum total IgE were determined by ELISA, the counting of the CD30 expressing cells was performed by flow cytometry. The results showed: (1)The level of IL 4 was increased significantly in patients with asthma attack compared with normal children ( P <0 01), and it lowered after remission though still higher than that of normal children ( P <0 01). On the other hand, the level of IFN γ was decreased significantly ( P <0 01), and it became higher after remission, but still lower than normal children( P <0 01). (2)The level of serum total IgE was increased significantly in patients with asthma attack compared with normal controls( P <0 01), and it lowered after remission, but still higher than normal controls( P <0 01).(3)The level of IL 4 was significantly positively correlated to serum IgE ( r =0 561, P <0 01). (4)CD30 antigen expression was much higher in asthma children. It was suggested that cytokine disturbances, due to changes of T cell subtypes, could be observed in children with asthma. These changes might be involved in the pathogenesis of asthma.
机构地区 解放军总医院
出处 《解放军医学杂志》 CAS CSCD 北大核心 2003年第6期560-561,共2页 Medical Journal of Chinese People's Liberation Army
关键词 哮喘 THL细胞 TH2细胞 白细胞介素4 干扰素Ⅱ型 抗原 CD30 asthma Th1 cells Th2 cells interleukin 4 interferon type Ⅱ antigens, CD30
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