摘要
采用HBVCTL表位多肽体外刺激或冲击免疫效 (E)、靶(T)细胞 ,以观察DNA疫苗诱导健康及HBV转基因 (Tg)小鼠细胞免疫效果。结果发现 ,DNA疫苗能有效诱导健康BALB/c小鼠CTL活性 ,活性强弱与E/T比值及其上清液中IFN γ分泌水平有一定关系。HBsAg表位多肽(pp2 0 )体外刺激DNA疫苗免疫组效应细胞 ,培养上清IL 1 2释放水平 (2 1 1 3± 39 8pg/ml)明显较对照组 (86 7±2 7 1 pg/ml)高(P <0 0 5 ,t=4 4 82 )。DNA疫苗免疫HBVTg小鼠诱导CTL活性 (1 2 7%± 6 7% )较蛋白疫苗免疫对照组(1 7%±3 2 % )高(P <0 0 1 ,t=3 6 2 9) ;其效应细胞体外受 pp2 0刺激后分泌IL 1 2水平 (4 0 0± 30 1pg/ml)明显高于同期空载体免疫对照组 (3 8±3 0 pg/ml,P <0 0 5 ,t=2 376 )。采用在体电脉冲法DNA疫苗接种的 5只HBVTg小鼠中 ,于 4周时有 2只血清HBsAg阴转 ,并于 8周时出现抗 HBs阳性 ,而对照组中无一例血清HBsAg发生变化。表明HBVDNA疫苗能有效诱导健康及HBVTg小鼠细胞免疫应答 。
The peptides from HBV cytotoxic T lymphocyte(CTL) epitope were used to either stimulate or impact the immune effector (E) or CTL target (T) cells respectively, in order to investigate the cellular immune response induced by DNA vaccination in both healthy and HBV transgenic (Tg) mice. It was found that HBV DNA based immunization could induce CTL activity in healthy BALB/c mice, with intensity correlated with the E/T ratio and IFN γ secretion level in its supernatants. The target cells hit by HBsAg CTL epitope peptide(pp20) could be lysed by the active CTL induced by HBV DNA vaccine encoding preS2 and HBsAg, while the cell lysis could not be observed in the target cells impacted by the HBcAg CTL epitope peptide (pp10). The supernatant IL 12 secretion level (211 3±39 8pg·ml -1 ) in the DNA vaccination group was significantly( P <0 05, t =4 482) higher than that (87 7±27 1pg·ml -1 ) in the pcDNA3 1 control group. In HBV Tg mice experiment, CTL activity (12 7±0 7%) induced by DNA vaccination was very significantly higher than that (1 7±3 2%) of the HBsAg proteineous vaccine control( P <0 01, t =3 629). The supernatant IL 12 secretion level (40 0±30 1pg·ml -1 ) of the DNA vaccine group was significantly( P <0 05, t =2 376) higher than that(3 8±3 0pg·ml -1 ) of the pcDNA3 1 control( P <0 05, t =2 376). By electroporation inoculation, the serum HBsAg of the 2 Tg mice out of the 5 became negative. At the 4 th week after DNA vaccination, the HBsAg serum conversion occurred in the 2 mice, but nothing happened among the 5 Tg mice in the control group at the 8 th week. The experimental results suggested that the HBV DNA vaccine we constructed could effectively induce cellular immune response in both healthy and HBV Tg mice, indicating that it was possible to use it as an anti HBV agent by improving the cellular immune function of the chronic carriers.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2003年第6期501-503,共3页
Medical Journal of Chinese People's Liberation Army
基金
国家高技术研究发展计划 (863计划 )基金资助课题 (编号2 0 0 1AA2 1 71 4 1 )
