摘要
测定比较了高产肌苷菌、低产肌苷菌和野生菌肌苷合成途径中PRPP转酰胺酶、sAMP合成酶、IMP脱氢酶和 5′ 核苷酸酶的比活以及活细胞的肌苷水解能力 ,进一步阐明了菌株产苷性能与肌苷合成途径关键酶活力间的密切关系。根据高产肌苷菌的酶学特性 ,确定了高产肌苷菌进一步基因工程改造的方向。研究表明酶学研究对有目的、有方向地进行高产菌株的选育工作具有重要意义。
The specific activities of key enzymes relating to the pathway of inosine synthesis of three different bacterial strains including high-yield, low-yield and wild strains were determined and compared systematically. A close relationship between inosine production and the specific activities of key enzymes was found. According to the enzyme characteristics of high-yield strain, suggestions on further strain improvement by modification of genetic engineering were proposed. Enzymology study is believed to be an effective way to make screening of high-yield strains more efficient.
出处
《微生物学报》
CAS
CSCD
北大核心
2003年第3期361-365,共5页
Acta Microbiologica Sinica
基金
上海市曙光计划资助项目 (0 1SG2 8)~~
