摘要
[目的]探讨恶性肿瘤患者体腔液细胞DNA倍体类型与细胞动力学参数之间的关系。[方法]采用流式细胞术对未进行化疗的91例恶性肿瘤患者体腔液细胞DNA含量进行了检测和分析。[结果]二倍体体腔液细胞的凋亡细胞检出率显著高于异倍体者(P<0.01),S鄄细胞比率(SPF)则与此相反(P<0.05)。在异倍体患者中,随体腔液异倍体细胞百分率的升高,凋亡细胞百分率与之呈显著的负相关(r=-0.8086,P<0.05),SPF则呈显著性正相关(r=0.9091,P<0.05)。不同数量异倍体克隆者的凋亡细胞检出率和SPF比率均有显著性差异(P<0.01)。SPF增高者的体腔液细胞的凋亡细胞和DNA异倍体检出率均低于SPF降低者(P<0.05)。[结论]恶性肿瘤能导致患者体腔液细胞的细胞凋亡水平显著降低,细胞增殖活性显著增加。而这些变化与体腔液细胞倍体类型、DNA异倍体细胞检出率和DNA异倍体克隆数均有十分密切的关系。
To investigate the relationship between DNA ploid types and parameters of cellular kinetics of coelom fluid cells in patients with malignancies.DNA contents in coelom fluid cells were measured by flow cytometry and analysed in91patients with malignancies before chemotherapy.The percentage of apoptosis in patients with diploidy(D)types was sig-nificantly higher than that in patients with heteroploidy(H)types(P<0.01),while the S-phase fraction(SPF)was contrary(P<0.05).A negative correlation between the percentage of H cells and that of apoptosis cells(r=-0.8086,P<0.05)in patients with H types was found,but positive correlation between H cells and SPF(r=0.9091,P<0.05).In patients with heteroploidy types,the percentage of apoptosis cells and SPF cells were significantly different among different number of DNA H colony(P<0.01).The percentage of apoptosis cells and H cells in patients with SPF increase were lower than those in patients with SPF decrease(P<0.05).[Conclusion]Malignant tumors may induce significant decrease of apoptosis percentage and remarkable increase of SPF in coelom fluid cells,which were closely related to DNA ploid types,the percentage of H cells and number of DNA H colony.
出处
《肿瘤学杂志》
CAS
2003年第3期125-127,共3页
Journal of Chinese Oncology
