摘要
为了解类植物乳杆菌L-XM1 plnBCD编码蛋白的生物学特性,并推测其功能,综合运用RPS-Blast、InterProscan和TMHMM Server等多种生物信息学软件预测分析plnBCD编码蛋白的理化性质、疏水性、磷酸化位点、保守性功能域及二级结构,并用Swiss-model同源建模。结果表明:plnB编码蛋白为疏水性蛋白,定位于细胞质膜上,有6个跨膜区、21个磷酸化位点和1个HATPase_c结构域,具有大多数组氨酸蛋白激酶的基本性质。其二级结构以α-螺旋和延伸链为主要构件,其空间结构与组氨酸蛋白激酶的结构特征一致。plnC及plnD编码蛋白均为亲水性蛋白,不具有跨膜区,定位于细胞质中,均具有1个REC和1个LytTR结构域。plnC及plnD编码蛋白与感应调节蛋白的空间结构具有较高的相似度。推测菌株L-XM1 plnBCD基因编码蛋白为群体感应调控系统组分。对plnBCD基因的生物信息学分析的研究可为发现新的群体感应系统和深入研究群体感应系统各组分的功能提供参考。
Bioinformatics softwares were used to predict and analyse the biological properties of plnBCD encoded proteins in Lactobacillus paraplantarumL-XM1,the functions of PlnBCD were also predicted.The physical and chemical properties,hydrohobicity,phospholyration sites,conserved domain,secondary structure information of the plnBCD encoded proteins were predicted using RPS-Blast,InterProscan,TMHMM Server,etc..Three-dimensional structure models were constructed using Swiss-Model homology modeling.The results showed that plnB encoded protein was a hydrophobic protein,and located in the plasma membrane,with six transmembrane regions and 21phosphorylation sites,one HATPase_c domain,which was consistent with the basic nature of most histidine protein kinase.α-helix and extended chains were the main secondary structure elements of plnB encoded protein.The spatial model of this protein was consistent with the histidine protein kinase.Both plnCand plnDencoded proteins were hydrophilic proteins,without transmembrane regions,located in the cytoplasm,and both were found with a REC domain and a LytTR domain.Both spatial models of plnC and plnD encoded proteins were similar to the response regulatory protein.Therefore,we predict that proteins encoded by plnBCDof strain L-XM1are components of quorum sensing system.The bioinformatics analysis of plnBCD provide a reference for finding a new quorum sensing system and a deep understanding of the functions of quorum-sensing components.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2014年第3期175-179,共5页
Journal of China Agricultural University
基金
国家自然科学基金资助项目(31071591)
关键词
类植物乳杆菌
生物信息学分析
同源建模
群体感应系统
Lactobacillus paraplantarum
bioinformatics analysis
homology modeling
quorum-sensing system
