摘要
作者用多阳离子化合物鱼精蛋白灌注大鼠肾脏,引起极似人类微小病变型肾病综合征病理改变.结合电镜观察,用图像分析仪及体视学原理分别对该模型肾组织胶体铁染色切片和电镜照片上足突平均厚度及足突占毛细血管的体积分数进行形态计量学研究.结果表明:用药组肾小球多阴离子明显减少;足突肿胀、融合、滤过裂隙变窄甚至消失。鱼精蛋白与多阴离子结合形成的电子致密颗粒,见于足突表面及基底膜内、外疏层,并以基底膜外疏层为著。显示肾小球多阴离子的分布特点.提示肾小球超微结构的改变可能系多阴离子被鱼精蛋白中和所致.
Perfusion of rat kidneyswith polycation protamine,resulted in patho-logic alterations of glomeruli very similarto those observed in minimal change neph-rotic syndrome in human. In the experi-mental model, colloidal iron stained kidneytissue sections were analyzed quantita-tivel by image analyzer and stereology incombination with the observation of othergolmerular ultrastructure. The mean thick-ness of foot processes and the foot processesmaking up the volume fraction of glomerularcapillary were determined. The studies sh-owed that there was a significant decreasein glomerular polyanions of kidneys perfu-sed with protamine as compared with thatof the control group(P<0. 01). The glomeru-lar alterations included swelling and fusionof foot processes and narrowing or loss offiltration slits. Electron-dense particles for-med by binding of prota mine to polyani-ons were seen primarily in the lamina raraexterna of glomerular basement membraneand also along the lamina rara interna andon the foot p rocess surface, which showedthe distribution feature of glomerularfilter. These results suggest that the ultra-structure alterations of glomeruli mayresult from the neutralization of polyanionsbyperfusion with protamine.
出处
《华西医科大学学报》
CAS
CSCD
1992年第3期272-275,共4页
Journal of West China University of Medical Sciences
关键词
鱼精蛋白
多阴离子
肾病综合征
Protamine
Polyanion
Image analyzer
Stereology
