摘要
目的 建立赤丹退黄颗粒中丹参酮ⅡA 的含量测定方法。方法 HPLC法 ,phenomenex(Luna)C18分析柱 ;甲醇 -水 (75∶2 5 )为流动相 ;检测波长 2 70nm。结果 丹参酮ⅡA 回归方程为Area=5 0 16 .18Amt.- 2 .16 5 0 ,r =0 .9998(n =6 ) ,在 0 .0 4~ 1.0 μg范围内呈线性关系 ,平均回收率为 10 2 .9% ,RSD为 1.0 7%。结论 方法简便、准确、快速 。
Aim To establish a HPLC method for the determination of tanshinone Ⅱ A in Chidan Tuihuang Keli.Method The tanshinone Ⅱ A was separated on a phenomenex(Luna) C 18 column at 270nm by MtOH-H 2O (75∶25) as a mobile phase.Result A linear relationship of tanshinone Ⅱ A was good at 0.04~1.0μg.The average recovery of tanshinone Ⅱ A was 102.9% with RSD 1.07%.Conclusion The method is simple,accurate and reliable for the determination of Tanshinone Ⅱ A in Chidan tuihuang Keli.
出处
《解放军药学学报》
CAS
2003年第2期149-151,共3页
Pharmaceutical Journal of Chinese People's Liberation Army