摘要
以肌苷产生菌BacillusSubtilisJSIM 1 0 1 9为出发菌株 ,根据B subtilis核酸代谢理论设计不同筛选模型 ,用物理、化学诱变剂对亲株进行诱变处理 ,先后有序地获得不同关键酶的缺失或回复即特殊的营养缺陷型以及抗某些代谢类似物的突变株 ,解除终产物对代谢物的抑制和阻遏 ,获得了几株黄嘌呤营养缺陷型并对 8氮鸟嘌呤具有抗性的突变株X 1 3等 ,获得的突变株经单菌分离后得到X 1 3 4 ,36℃培养 72h在培养基中最高积累 1 2 4 3g/L腺苷。
According to the theory of nucleic acid metabolism, the different select models were designed By means of physical and chemical derived to the production of inosine Bacillus Subtilis JSIM 1019, we have got the mutative strains of auxotroph of different component, the mutative strains lacking of relating enzyme, the mutative strains resisting some metabolic analog in turn Restrain of the production to metabolite has been relieved Two mutative strains of auxotroph xanthine, X 13 and so on were obtained And it also has resistance to 8 azaguanine After the mutative strains had been separated into the single srain respectively, we got X 13 4 the maximum accumulation of adenosine reachs 12 43g/L in substratum cultivated for 72 hours at 36℃
出处
《微生物学通报》
CAS
CSCD
北大核心
2003年第2期52-56,共5页
Microbiology China
基金
江苏省社会发展资助项目 (No BS970 80 )
