摘要
用诱变剂N 甲基 N 亚硝基脲 (NMU)诱变向日葵种子时 ,温度、2 ,4 二硝基苯酚 (DNF)、利福平 (R)、KT、硝酸铵(AA)、二氨基苯甲酸 (DABA)和处理时间都会影响NMU对向日葵叶绿体的突变诱导效果。突变诱导的最佳温度为 15℃ ,2 ,4 二硝基苯酚、利福平、硝酸铵和二氨基苯甲酸明显降低花叶植株的频率 ,而KT则增加叶绿体突变的频率 ,在 0 .0 15 %KT作用下 ,叶绿体突变频率由 (8.5± 2 .9) %提高到 (2 5 .0± 4 .2 ) %。咖啡因 (COF)对诱导叶绿体突变没有任何作用。
s Such factors as temperature, time interval between the end of treatment and the beginning of wax of treatment seeds, as well 2,4 dinitrophenol, rifampicin, kinetin, NH 4NO 3, P amino benzoic acid have been shown to modify the mutagenic effect of N methyl N nitrosourea on chloroplasts in sunflower. Optimal temperature of induction mutation is 15℃, 2,4 dinitrophenol, rifampicin, NH 4NO 3 and P amino benzoic acid all decreased plastid mutation, while kinetin reversed. At concentration 0.015% KT, plastid mutation increased from (8.5±2.9)% to (25.0±4.2)%. Caffeine has been found to have no influence on induction of plastid mutations.
出处
《植物生理学通讯》
CSCD
北大核心
2003年第2期105-108,共4页
Plant Physiology Communications
