摘要
目的 探讨地塞米松 (DEX)治疗与细菌性脑膜炎脑源性神经营养因子 (BDNF)及其受体TrkB基因表达的关系。方法 建立细菌性脑膜炎模型 ,分别用抗菌药物及抗菌药物加DEX治疗 ,用原位杂交检测脑组织BDNFmRNA和TrkBmRNA的表达。结果 单独使用抗菌药物治疗后BDNFmRNA和TrkBmRNA表达均低于感染后 5d组的水平 (P <0 .0 5 ) ,并以BDNFmRNA下降更明显 (P <0 .0 1) ,而使用DEX与抗菌药物联合治疗后 ,BDNFmRNA和TrkBmRNA表达回到较高水平 (P <0 .0 1) ,BDNFmRNA达到感染后 5d组水平 (P >0 .0 5 ) ,TrkBmRNA则超过感染后 5d组水平 (P <0 .0 5 )。结论 DEX则可能通过上调内源性BDNFmRNA和TrkBmRNA表达 ,有利于抵御细菌性脑膜炎脑损伤。
Objective To investigate the relationship between dexamethasone and brain derive neurotrophic factor gene in experimental bacterial meningitis. Methods To construct the models of bacterial meningitis in 3 weeks old rats(n=33),then 23 of them were administed antibiotic and antibiotic plus dexamethasone respectively. BDNF mRNA and TrkB mRNA in brain was detected by in situ hybridization methods,respectively. Results After administration antibiotics, the expression of BDNF mRNA and TrkB mRNA in brain tissue were less than that in brain after infection 5 d(P< 0.05), especially level of BDNF mRNA was much lower (P< 0.01), and after administration by antibiotics plus DEX, it was greater than that in brain after administration by antibiotics(P< 0.01), the expression of BDNF mRNA was the same as that in brain at 5 d after infection(P> 0.05), the expression of TrkB mRNA was stronger than that in brain at 5 d after infection(P< 0.05). Conclusions The results support the hypothesis that DEX might be propitious to resist brain injury in bacterial menningitis by up-regulation the expression of BDNF mRNA and TrkB mRNA.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
2003年第2期128-131,共4页
Chinese Journal of Infectious Diseases
