摘要
目的 :肝癌高低转移细胞株的基因表达谱的比较。方法 :应用cDNAmicroarray技术 ,对肝癌高低转移细胞株的基因表达谱进行比较 ,并应用RT PCR对部分差异表达的基因进行了验证。结果 :肝癌高转移细胞株与低转移细胞株相比 ,有 384个基因存在差异表达 ,其中 1 2个是位于染色体 8p上。选取 4个基因用RT PCR验证 ,结果与cDNAmicroarray结论基本一致。 结论 :肝癌高低转移细胞株基因表达存在明显差别。染色体
Purpose:To study the difference in gene expression profile between HCC cell strains with high and low metastatic potential.Methods:The gene expression profile of high and low metastatic HCC cell strains were analyzed by cDNA microarray, and genes with differential expression were further verified by RT PCR.Results:384 differentially expressed genes were detected between high and low metastatic HCC strains, making up 32% of the total genes studied. RT PCR analysis on 4 genes chosen demonstrated the same results as those revealed by the array technique.Conclusions:Obvious difference in gene expression patterns was observed between high and low metastatic HCC cell strains, chromosome 8p may contain metastasis promote and inhibitory genes.
出处
《中国癌症杂志》
CAS
CSCD
2003年第2期97-101,共5页
China Oncology
基金
国家自然科学基金 ( 30 0 0 0 0 75)
教育部科学技术重大项目( 2 2 5A)
上海市启明星计划 ( 0 0QB14 019)
复旦大学MedX基金资助
关键词
肝癌
转移
高转移细胞株
低转移细胞株
liver neoplasms
hepatocellular carcinoma
metastasis
cDNA microarray
chromosome 8p
cultured tumor cells
