摘要
随着生物人工肝和肝细胞移植研究的开展 ,对肝细胞的需求不断增加 ,迫切需要有效的肝细胞长期保存方法以促进其在临床上的应用。低温保存是目前保存肝细胞的重要方法 ,其影响因素较多 ,主要有低温保存肝细胞的形式冻存液的组成、降温、复温速度 ,复苏肝细胞的功能检测及应用范围等。
With the development of bioartificial liver and hepatocytes transplantation,the demand of hepatocytes is increasing rapidly,and it is necessary of find an effective method of long term preserving hepatocytes.Now hypothermic preservation is an important measure of preserving hepatocytes.But there are several affected agents,such as the pattern of preserving hepatocytes,conformation of freezing medium,the rate of reducing and rewarming temperature,the functional detection and application of hepatocytes after cold storage.The text will summarize latest progress on it.
出处
《国外医学(生物医学工程分册)》
2003年第2期85-89,共5页
Foreign Medical Sciences(Biomedical Engineering Fascicle)
关键词
肝细胞
低温保存
冻存保护刑
生物人工肝
hepatocytes
hypothermic preservation
cryoprotective agent
bioartificial liver