摘要
为给四川小麦品质育种提供参考信息,利用7个HMW-GS、17个LMW-GS和1个1B/1R易位的特异分子标记,对105份2000年后育成的四川小麦品种进行上述基因检测。结果表明:(1)针对HMWGS,在Glu-A1位点,含Ax2*的品种有2份,频率为1.9%;在Glu-B1位点,含Bx7、Bx20、Bx17、By8和By9的品种分别有73、26、4、45和30份,频率分别为69.5%、24.8%、3.8%、42.9%和28.6%,未检测到含Bx7OE的品种;在Glu-D1位点,含Dx5的品种有65份,频率为61.9%。(2)针对LMW-GS,在Glu-A3位点,含Glu-A3a、Glu-A3b、Glu-A3c、Glu-A3d和Glu-A3f的品种分别有2、2、63、29和9份,频率分别为1.9%、1.9%、60.0%、27.6%和8.6%,未检测到含Glu-A3e和Glu-A3g的品种;在Glu-B3位点,含Glu-B3b、Glu-B3d、Glu-B3f、GluB3g和Glu-B3i的品种分别有18、10、1、75和1份,频率分别为17.1%、9.5%、1.0%、71.4%和1.0%,未检测到含Glu-B3a、Glu-B3c、Glu-B3e和Glu-B3h的品种。(3)含1B/1R易位的品种有36份,频率为34.3%。(4)组合6种和5种以上优质基因的品种分别有2份(频率为3.8%)和15份(频率为14.3%)。可利用这些品种作为亲本,在四川小麦品质育种中逐步导入优质基因Ax2*、Bx7、By8、Dx5、Glu-A3d、Glu-B3d、Glu-A3b和Glu-B3b,并淘汰1B/1R易位,优化四川小麦面筋优质基因组成。
The composition of high and low molecular weight glutenins and 1B/1Rtranslocation is key information on wheat quality breeding.Using a total of 25 DNA molecular markers,including 7markers specific to HMW-GS,17 to LMW-GS and 1to 1B/1Rtranslocation,105 Sichuan wheat cultivars released since 2000 were identified.The results showed that:(1)For HMW-GS,2cultivars contained Ax2*at Glu-A1 locus,with a frequency of 1.9%.At Glu-B1 locus,73cultivars had Bx7,26 carried Bx20,4possessed Bx17,45 had By8 and 30 carried By9,with a percentage of 69.5%,24.8%,3.8%,42.9% and 28.6%,respectively.At Glu-D1 locus,65 cultivars contained Dx5,with a frequency of 61.9%.(2)For LMW-GS,the genes frequencies of the Glu-A3 a,Glu-A3 b,Glu-A3 c,GluA3d,and Glu-A3 fat Glu-A3 locus were 1.9%,1.9%,60.0%,27.6% and 8.6%,respectively.At Glu-B3 locus,the frequencies of the Glu-B3 b,Glu-B3 d,Glu-B3 f,Glu-B3 gand Glu-B3 iwere 17.1%,9.5%,1.0%,71.4% and 1.0%,respectively.(3)Thirty-seven cultivars with 1B/1Rtranslocation were detected,with a frequency of 34.3%.(4)2(3.8%)and 15(14.3%)cultivars were found with a combination of 6or 5genes of Ax2*,Bx7,By8,Dx5,Glu-A3 d,Glu-B3 d,Glu-A3 b,Glu-B3 band non-1B/1Rtranslocation.These cultivars can be used as parents in wheat breeding for introducing genes with better quality potential related to gluten quality of Sichuan wheat cultivars.
出处
《麦类作物学报》
CAS
CSCD
北大核心
2014年第12期1619-1626,共8页
Journal of Triticeae Crops
基金
四川省财政基因工程论文基金项目
四川省农作物育种攻关项目
国家"863"计划项目(2012AA101105)
国家自然科学基金项目(NSFC31271724)
四川省科技支撑项目(2011NZ0098-16)
四川省青年科技创新研究团队项目(2014TD0014)
