摘要
对牵牛花花蜜中细菌进行分离、纯化和培养,共获得29株细菌菌株,以传统方法通过形态学、培养性状以及生理生化特征对其进行鉴定,结果表明:29株细菌分别属于芽孢杆菌属(Bacillus sp.)、泛生菌属(Pantoea sp.)、柠檬酸细菌属(Citrobacter sp.)、微杆菌属(Microbacterium sp.)、假单胞菌属(Pseudomonas sp.)、土壤杆菌属(Agrobacterium sp.)、鞘氨醇单胞菌属(Sphingomonas sp.)。芽孢杆菌分离获得的几率最高。对牵牛花花蜜中细菌多样性进行研究,可为花蜜中细菌多样性研究提供基础资料,为进一步研究蜜源植物花蜜中细菌对蜜蜂体内细菌结构的影响提供一定的参考数据。
Based on traditional morphology, cultivated character, physiological and biochemical method, we obtained 29 bacterial isolates from nectar of Pharbitis nil.The results indicated 29 isolates were related to 7genu,includi,Bacillus,Pantoe,Citrobacter,Microbacterium,Pseudomonas, Agrobacterium and Sphingomonas. The largest number species was Bacillus sp. This paper provided basic data for bacterial diversity in nectar and make further study to prove whether structure of bacteria of honey bee was affected by nectar bacteria.
出处
《蜜蜂杂志》
2014年第6期1-5,共5页
Journal of Bee
基金
国家农业产业技术体系(蜜蜂)(CARS-45-ksj14)
关键词
牵牛花
花蜜
细菌多样性
鉴定
Pharbitis nil
nectar
bacterial diversity
identification
