摘要
In thes paper the authors used the Horseradish peroxidase labelledstaphylococcal protein A(HRP—SPA)in ELISA,for the detection of Clo-norchis sinensis infection.Serum tests were made on 116 confirmed cases ofclonorchiasis,103(88.8%)of them showed positive,while only 6(4.4%)werepositive among 138 healthy people.Samples were collected on filter paperstrips,111(95.7%)cases were positive among 116 comfirmed cases tested,but only 2(1.5%)were positive out of 138 healthy persons.The resultswere similar to those obtained by sheep antihuman IgG.Animal experimentalso showed that the SPA—ELISA can be used for the diagnosis ofclonorchiasis.In an endemic area,stool egg positive rate was 8.8%(62/703).whenchecked with SPA—ELISA,the rate of conformity in both filter paperstrips and stool examinations was 90.3(56/62).Among 641 serum testsfrom individuals negative in stool examinations,only 35(5.5%)reactedpositively.The authors suggested—that SPA—ELISA with soluble Clo-norchis antigens could be used in a large scale seroepidemiological surveyin endemic areas.
In thes paper the authors used the Horseradish peroxidase labelled staphylococcal protein A(HRP—SPA)in ELISA,for the detection of Clo- norchis sinensis infection.Serum tests were made on 116 confirmed cases of clonorchiasis,103(88.8%)of them showed positive,while only 6(4.4%)were positive among 138 healthy people.Samples were collected on filter paper strips,111(95.7%)cases were positive among 116 comfirmed cases tested, but only 2(1.5%)were positive out of 138 healthy persons.The results were similar to those obtained by sheep antihuman IgG.Animal experiment also showed that the SPA—ELISA can be used for the diagnosis of clonorchiasis. In an endemic area,stool egg positive rate was 8.8%(62/703).when checked with SPA—ELISA,the rate of conformity in both filter paper strips and stool examinations was 90.3(56/62).Among 641 serum tests from individuals negative in stool examinations,only 35(5.5%)reacted positively.The authors suggested—that SPA—ELISA with soluble Clo- norchis antigens could be used in a large scale seroepidemiological survey in endemic areas.
出处
《潍坊医学院学报》
1985年第2期146-151,共6页
Acta Academiae Medicinae Weifang
