摘要
目的 研究复方中药对肝硬化大鼠血浆及肝组织内皮素 1(endothelin 1,ET 1)和肝组织ET 1mRNA表达的影响。方法 采用放射免疫法测定血浆和肝组织ET 1水平 ,逆转录多聚酶链反应 (RT PCR)测定大鼠肝组织ET 1mRNA表达水平。结果 模型组血浆 (171 99± 2 7 86pg/ml)和肝组织ET 1(80 4 8± 2 1 4 5 pg/ml)较对照组 (114 33± 17 34pg/ml,5 3 2 5± 13 6 4pg/ml,P <0 0 5 )均显著升高 ,肝组织ET 1mRNA表达也较对照组 (0 4 6 74± 0 10 4 5vs.0 14 13± 0 0 2 97,P <0 0 5 )显著升高。治疗组血浆ET 1(12 9 80± 13 15 pg/ml)和肝组织ET 1mRNA表达 (0 30 4 0±0 0 813)均显著低于模型组 (171 99± 2 7 86 pg/ml,0 4 6 74± 0 10 4 5 ,P <0 0 5 )。而治疗组肝组织ET 1(77 36± 18 2 7pg/ml)与模型组 (80 4 8± 2 1 4 5 pg/ml)相比无显著差异。 结论 复方中药能显著降低肝硬化大鼠肝组织ET 1mRNA的表达及血浆ET 1水平。
ObjectiveTo investigate the effects of compound Chinese herbs on plasma and hepatic endothelin-1 (ET-1) and hepatic tissue ET-1 mRNA expression in CCl4-induced cirrhotic rats. MethodsThe plasma and hepatic levels of ET-1 were measured with radioimmunoassay and hepatic tissue ET-1 mRNA expression determined with RT-PCR. ResultsThe plasma and hepatic levels of ET-1 were significantly higher in cirrhotic rats than in controls (171.99±27.86 pg/ml vs. 114.33±17.34 pg/ml, 80.48±21.45 pg/ml vs. 53.25±13.64 pg/ml, P<0.05). Meanwhile, the hepatic tissue ET-1 mRNA expression was markedly higher in the cirrhotic rats than in those controls (0.4674±0.1045 vs. 0.1413±0.0297, P<0.05). The plasma level of ET-1 and hepatic tissue ET-1 mRNA expression were significantly lower in cirrhotic rats receiving treatment of compound Chinese herbs than in those not receiving the treatment (P<0.05). However, there was no significant difference in hepatic level of ET-1 between these 2 groups of rats (P>0.05). ConclusionsCompound Chinese herbs can significantly decrease the hepatic tissue ET-1 mRNA expression and plasma level of ET-1 in CCl4-induced cirrhotic rats.
出处
《中华肝胆外科杂志》
CAS
CSCD
2003年第4期225-227,共3页
Chinese Journal of Hepatobiliary Surgery
