摘要
目的 观察醋酸铅染毒对大鼠海马和小脑c fos基因表达及学习记忆功能的影响 ,同时观察阿胶、天麻的拮抗作用。方法 大鼠经亚慢性醋酸铅染毒 (高剂量 0 .2g·kg- 1 ·d- 1 ,低剂量 0 .1g·kg- 1 ·d- 1 ) ,同时设立天麻 (4g·kg- 1 ·d- 1 )、阿胶 (1g·kg- 1 ·d- 1 )单独和联合作用组 ,监测各组血铅浓度 ,观察各组大鼠学习记忆功能的变化 ,最后利用原位杂交技术观察学习记忆过程中各组海马CA3区及小脑c fosmRNA表达状况的变化。结果 (1 )高、低剂量的铅染毒均使大鼠血铅浓度增加 ,与对照组比较 ,差异均有显著性 (P <0 .0 1 )。 (2 )Y型迷宫试验中 ,染铅使大鼠学习达标前所受电击次数增加 ,与对照组比较 ,差异有显著性 (P <0 .0 1 ) ;天麻和阿胶单用可减少染铅大鼠学习达标前所受电击次数 ,与染铅组比较 ,差异有显著性 (P <0 .0 5) ,二者联合应用较之单独应用使电击次数减少更明显 ,与染铅组比较 ,差异有显著性 (P <0 .0 1 )。 (3)低铅 +天麻 +阿胶组标本切片上海马CA3区着色较深的c fos阳性细胞最为密集 ,与对照组接近 ,高铅 +天麻 +阿胶组也有较多染色较深的c fos阳性细胞 ,但分布不及低铅 +天麻 +阿胶组密集 ,高铅组、低铅组则罕见c fos阳性细胞 ,各天麻。
Objective To observe the effect of lead on the expression of c fos mRNA in hippocampus and cerebellum and the ability of learning and memory in rat,and to observe the antagonistic action of Gastrodia elata and E gelatin on the effect mentioned above. Methods Rats were exposed to lead acetate(0.2 g·kg -1 ·d -1 and 0.1 g·kg -1 ·d -1 ).The single and combined administration of Gastrodia elata(4 g·kg -1 ·d -1 ) and E gelatin(1 g·kg -1 ·d -1 ) were conducted at the same time.Blood lead concentration in rats were measured.The ability of learning and memory by Y maze test were examined.The expression of c fos mRNA in hippocampus and cerebellum during Y maze test were observed by in situ hybridization(ISH). Results (1)Blood lead concentrations were significantly increased in both high and low doses of lead exposed rats( P <0.01).But there were no differences among high dose groups or low dose groups( P >0.05).(2)In Y maze test,the number of electric stroke druing learning in lead exposed group was significantly increased( P <0.01) while that in Gastrodia elata and E gelatin groups was significantly decreased( P <0.05),and that in combined use group was more significantly decreased( P <0.01).(3)There were much more deep colored c fos positive cells in CA3 area of hippocampus in low dose of Pb exposed with Gastrodia elata+E gelatin group.These cells were also found in high Pb with combined use or single use group,but which were not so densely distributed as the former.And they were hardly found in high and low doses of Pb alone groups.The changes of expression of c fos cells in cerebellam were similar to those in hippocampus. Conclusion The down regulation of c fos expression may be one of the molecular mechanism of lead induced impairment of learning and memory.Gastrodia elata and E gelatin may antagonize the effect of lead on c fos expression,and combined use of both drugs may potentiate the antagonism.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2003年第2期128-131,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
山东省博士基金资助项目 ( 94 7536 )
