摘要
提出用固相萃取 -高效液相色谱法同时测定饲料中微量克伦特罗和沙丁胺醇的新方法。选用 Dikma Diamonsil C18-ODS分析柱 (2 0 0× 4.6mm,5μm) ,乙腈 -0 .0 1mol/ L KH2 PO4 (p H 3 .0 )作流动相 ,应用波长编程进行检测。克伦特罗和沙丁胺醇的线性范围为 0 .1～ 1 0 0μg/ m L,相关系数分别为 0 .99999和 0 .99977,检出限分别为0 .3 1 ng/ m L和 0 .2 4ng/ m L ,回收率分别为 91 .2 %～ 92 .0 %和 91 .9%～ 93 .0 % ,相对标准偏差分别是 1 .2 0 %～ 2 .0 5%和 1 .2 9%～ 2 .51 %。
A procedure for the simultaneous determination of clenbuterol and salbutamol in feed samples by high performance liquid chromatography/solid phase extraction has been proposed. The analysis was carried out with Diamonsil C 18 column, acetonitrile/water (0.01 mol/L KH 2PO 4,pH3.0)as mobile phase and wavelength program. The range of linear relationship of clenbuterol and salbutamol was 0.1～100 μg/mL ( r =0.99999 and 0.99977). The minimum detectable limit was 0.31 ng/mL and 0.24 ng/mL, respectively. The standard recovery was 91.2%～92.0% and 91.9%～93 0%, respectively. RSD was 1.20%～2.30% and 1.29～2.58%, respectively.
出处
《分析科学学报》
CAS
CSCD
北大核心
2003年第2期145-147,共3页
Journal of Analytical Science
