摘要
目的 评价辛基酚 (OP)的类雌激素活性 ,并对其作用机制进行初步研究。方法 采用了体外MCF 7细胞增殖试验检测了OP的类雌激素活性 ,并用生长曲线分析、细胞周期分析、他莫昔芬拮抗试验和凋亡检测对其作用机制进行了初步探讨。结果 OP有明显刺激MCF 7细胞增殖的类雌激素活性 ;细胞周期分析OP、E2 组细胞增殖指数均明显高于溶剂对照组 ;OP、E2 刺激MCF 7细胞增殖的类雌激素活性可被Tam拮抗 ;OP、E2 均能明显抑制MCF 7细胞凋亡的发生。结论 OP具有刺激MCF 7细胞增殖的类雌激素活性 ,其机制可能是与雌激素受体结合后 ,影响细胞增殖及细胞凋亡过程 。
Objective To observe the estrogenic activity of octylphenol(OP) in vitro and to conduct a preliminary study of its mechanism. Methods The estrogenic activity of OP was detected by cell proliferation test of MCF 7 cells in vitro and the mechanism was preliminarily studied by growth curve analysis, cell cycle analysis, tamoxifen(Tam) antagonistic test and apoptosis detection. Results OP was found to have estrogenic activity to stimulate the proliferation of MCF 7 cells. Cell cycle analysis revealed that the cell proliferation indexes of OP and 17β estradiol(E 2) were higher than those of alcohol. The estrogenic activities of OP and E 2 to stimulate the proliferation of MCR 7 could be antagonized by Tam. Both OP and E 2 could inhibit the cell apoptosis of MCF 7 cells. Conclusion OP possesses estrogenic activity to stimulate the proliferation of MCF 7 cells. The mechanism may be due to binding to the estrogen receptor, which may have effect on cell proliferation and apoptosis.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2003年第8期673-675,共3页
Journal of Third Military Medical University
基金
国家自然科学基金资助项目 ( 3980 0 12 7)
