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Optimization of Propionic Acid Feeding for Production of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in Fed-Batch Culture of Ralstonia eutropha 被引量:3

Optimization of Propionic Acid Feeding for Production of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in Fed-Batch Culture of Ralstonia eutropha
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摘要 The feeding method of propionic acid for production ofpoly(3-hydroxybutyrate-co-3-hydro xyvalerate) [P(3HB-co-3HV)] by fed-batch culture of Ralstoniaeutropha was optimized to achieve high cell density and high 3HV yield. Effects of different feedingstrategies of propionic acid on the production of P(3HB-co-3HV) were investigated. A decline ofspecific synthesis rate of copolymer and the yield of 3HV unit from propionic acid were observed dueto the propionic acid accumulation in culture broth when the feeding solution with highP/G(propionic acid to glucose) ratio was employed. It was further confirmed by controlling propionicacid concentration at a low level in the separate feeding of propionic acid. An optimal feedingstrategy was demonstrated to reduce the propionic acid accumulation. The cell concentration,P(3HB-co-3HV) productivity and 3HV unit fraction reached to 163.9kg·m^(-3), 1.8kg·m^(-3)·h^(-1),and 10.6%(by mass), respectively, resulting in a yield of 0.33g HV per g propionic acid. The feeding method of propionic acid for production of poly (3-hydroxybutyrate-co-3-hydro xyvalerate)[P(3HB-co-3HV)]by fed-batch culture of Ralstonia eutropha was optimized to achieve high cell density and high 3HV yield,Effects of different feeding strategies of propionic acid on the production of P(3HB-co-3HV) were investigated,A decline of specific synthesis rate of copolymer and the yield of 3HV unit from propionic acid were observed due to the propionic acid accumulation in culture broth when the feeding solution with high P/G(ropionic acid to glucose) ration was employed,It was further confirmed by controlling propionic acid concentration at a low leve in the separate feeding of propionic acid.An optimed feeding strategy was demonstrated to reduce the propionic acid accumulation ,The cell concentration,P(3HB-co-3HV) productivity and 3HV unit fraction reached to 163.9 kg.m^-3,1.8kg.m^-3.h^-1,and 10.6%(by mass),respectively,resulting in a yield of 0.33g HV per g propionic acid.
出处 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2003年第2期220-223,共4页 中国化学工程学报(英文版)
关键词 biodegradable polyester feeding strategy P(3HB-co-3HV) propionic acid ralstonia eutropha R.eutropha 批式流加培养 生产 羟基丁酸 丙酸 混合聚酯 进料 优化 发酵 PHA
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