摘要
在对玉米等主要农作物进行病理及致病毒素毒理分析时,经常选用根冠脱落细胞作为试材.许多学者认为这些脱落的细胞处于垂死状态,然而,后来的一些研究肯定了Knudson的脱落根冠细胞具有活力的观点[1].
The detached root cap cells from maize seedlings comprised of round-shaped cells, ellipse-shaped cells and longitude-shaped cells. By using fluorescein-iso-thiocyanate-phalloidin(FITC-Ph) as fluorescent probe and treatment with cytochalasin B ( CB ) or HMC toxin, a host-specific toxin from Bipolaris (Helminthosporium ) maydis race C , the distribution and variation of microfilaments (MFs) in detached cells were investigated. The results were as follows ( i ) the round-shaped cells had intense fluorescence, but the network of MFs was not distinct. There was clear MFs network in the cytoplasm of both ellipse-shaped and longitude-shaped cells. The distribution of MFs in detached cells seemed to be relevant to their shape and vigor. ( ii ) the fluorescence of detached cells of Charrua cytoplasmic male sterility(cms-C) maize was decreased after treatment with HMC-toxin. The cause of this outcome was unclear. We only obtained the pictures of the distorted protoplast membrane and dead cells owing to treatment with HMC-toxin. The distribution of MFs of detached cells of Normal(N) cytoplasmic maize was not affected by HMC-toxin, and their protoplasts shank slightly. ( iii ) CB could change the distribution of MFs in detached cells of both cms-C maize and N maize to disordered arrangement.
出处
《实验生物学报》
CSCD
北大核心
2003年第2期149-154,共6页
Acta Biologiae Experimentalis Sinica
基金
河北省自然科学基金(No.300163)
