摘要
通过单细胞分离(克隆)培养和局部淋巴结转移灶筛选的方法,建立两种不同淋巴转移能力HepA肝癌细胞亚系,分别命名为HepA-H和HepA-L,并比较其生长、游走和贴壁能力等生物学特性。瘤细胞接种于小鼠足垫后,同侧腘窝淋巴结转移率,HepA-H为83.3%,HepA-L为16.7%,两者具有显著差异(p<0.01)。两种细胞亚系均未见肺、肝、脾、肾等脏器转移灶。体外实验显示,HepA-H的生长、游走和贴壁能力均强于HepA-L。HepA肝癌不同淋巴转移能力亚系的建立,为研究肿瘤经淋巴管转移机理提供了良好的肿瘤淋巴转移动物模型。
The aim of the experiment is to establish two murine hepatocarcinoma (HepA cell line) sub-strains with different lymphatic metastatic ability and compare their biological characteristics. Two murine hepatocarcinoma substrains, HepA-H and HepA-L, were established by the cell cloning and the selection way of lymph node metastasis. After tumor cells were injected subcutaneously to foot pad of NIH mice, the popliteal lymph node metastatic rates of HepA-H and HepA-L were 83.3 % and 16.7% respectively, which has statistical significance (p<0. 01). There were no metastatic lesions were found in other organs, such as lung, liver, spleen and kidney. In vitro , HepA-H showed a higher ability in growth, migration and adhesion than that of HepA-L. The establishment of two murine hepatocarcinoma substrains provides a useful animal model for the study of mechanism of the lymphatic metastasis.
出处
《实验生物学报》
CSCD
北大核心
2003年第2期99-104,共6页
Acta Biologiae Experimentalis Sinica
