摘要
首次成功地进行了人工条件下波叶仙鹤薛(Atrichum undulatum(Hedw.)P.Beauv)单细胞孢子的培养,并详细研究、拍摄了其植物体(配子体)形态发育的全过程。孢子无后熟或休眠期,3～4天即可萌发,萌发率可达95%以上;培养10天左右绿丝体系统、轴丝体系统及假根开始分化,约40天开始出现芽体原基,50天芽体陆续形成,80天左右植物体完成营养生长,开始性器发育。初生假根发育不良,基原细胞不分裂,由绿丝体产生轴丝体的方式复杂,轴丝体细胞分裂面有球形和斜向、横向三种,芽原基分化时,首先在轴丝体上产生光合能力很强的细胞群,其基部产生芽原基,芽原基的分化成功率仅有50%左右,芽体形成后,原丝体系统多枯萎或特化为假根。并就波叶仙鹤藓的形态发育、生理生态、生殖和进化等方面也进行了理论探讨。
Artificial culture of unicell spore of Atrichum undulatum (Hedw. ) P. Beauv is performed successfully in this paper, and the authors research the whole process of that and take the photoes. through the research, we find that its spores have no post - maturation or domant period. They can germinate after 3-4 days and the rate of germination is over 95% . About 10 days later, its chlorone-mata, caulonemata and rhizoides begin to differentiate, almost on the 40th day bud primordium ap-pears, and then, around the 50th day, bud forms gradually. At last, nearly the 80th day, the whole plant finishes its vegetive growth and starts development of sexual organs. Its primary rhizoides devel-op poorly, and basai celis have no division, the way of chloronema's generating caulonema is complex and caulonema cell has three kinds of diastemas; spherical, declinate and transuerse, at the beginning of the differentiation of bud primordium, caulonema generates cell population that have high photosyn-thetic capacity, and bud primordium on its base. The rate of successful differentiation of the bud primordium is about only 50% , once bud forms, protonemata always perishes and specializes in rhizoides. The research discussed the morpha development, phyoiological ecology,generation, evolution and so on.
出处
《植物研究》
CAS
CSCD
北大核心
2003年第2期159-163,T005,共6页
Bulletin of Botanical Research
基金
国家攻关项目资助
项目号:2001BA510B-01-04-04
