摘要
目的 了解IL 13在哮喘发作时水平及其与IgE的相互关系 ,从而探讨IL 13在哮喘发病中的作用。方法 分别用逆转录 多聚酶链反应 (RT PCR)及ELISA法检测哮喘患儿外周血单个核细胞 (peripheralbloodmononuclearcell,PBMC)在PHA刺激下IL 13mRNA表达及培养上清中IL 13蛋白水平的变化 ,并分析IL 13蛋白水平与IgE的相关性。 结果 哮喘发作患儿IL 13mRNA表达相对强度和蛋白质水平显著高于正常对照组 ,差异有显著性 (P <0 .0 5 ) ;哮喘发作患儿PBMC培养上清IL 13与血浆总IgE水平呈显著正相关 ,相关系数 (r)为 0 .5 93。结论 在哮喘发作期IL 13的产生明显增高 ,将IL 13水平的变化作为观察病情活动状态的一个免疫学指标具有一定的临床意义。是否能将IL
Objective To explore the effect of IL 13 in asthma pathogenesis. Methods RT PCR was used to detect the expression levels of IL 13mRNA in PBMC stimulated with PHA and ELISA was used to detect the levels of IL 13 in culture supernatants of PBMC stimulated with PHA and the level of plasma total IgE. Results Compared to the control group , in asthmatic children (1)The expression level of IL 13mRNA in PBMC stimulated with PHA was elevated significantly; (2) The levels of IL 13 in culture supernatants stimulated with PHA increased significantly; (3)The plasma total IgE level was higher than that of control′s; (4) There was a significantly positive correlation of IL 13 in culture supernatants with plasma total IgE; Conclusions IL 13 may play an important role in asthma pathogenesis and it seems to be an effective method to treat asthma using IL 13 monoclonal antibody.
出处
《重庆医学》
CAS
CSCD
2003年第4期391-393,共3页
Chongqing medicine
基金
国家自然科学基金资助项目 (30 2 71 380 )
